Determination of 2-undecanone in rat plasma and tissue by a high peormance liquid chromatography method: An application for the pharmacokinetic and tissue distribution research

The purpose of this study was to establish a reliable high peormance liquid chromatography (HPLC) method to investigate the pharmacokinetic and tissue distribution profiles of 2-undecanone. A LC2010AHT HPLC system coupled with UV detector was employed and the separations were performed on a ZORBAX Extend C18 analytical column. The mobile phase was a mixture of 50% acetonitrile and 50% water at a flow rate of 0.7 mL/min with isocratic elution. The method was validated within the concentration range 0.08-5.00 μg/mL for plasma samples, and 0.04-4.00 μg/g for tissue samples, the calibration curves were linear with correlation coefficients >0.99. The limit of detection (LOD) was 0.03 μg/mL or 0.02 μg/g (three times signal=noise ratio). Intra- And inter-day precisions expressed as the relative standard deviation (RSD) for the method were 1.95-3.89% and 2.43-4.23%, respectively. The method recoveries for all samples were >80%. The main pharmacokinetic parameters obtained were t _max=(0.32±0.10) h, C_max=(6.47±1.91) μg/mL, AUC_0→=(9.81±3.11) (μg/mL) · h, and Ka=(5.30± 1.73) · h^-1. The concentrations of 2-undecanone in liver (5.50±0.26 μg/g) and spleen (3.90±0.22 μg/g) were higher than in the other investigated tissues.