TY - GEN
T1 - Detection of pathogens using bio-activated nickel hexacyanoferrate film coated interdigitated microelectrodes
AU - Pires, Nuno M.M.
AU - Dong, Tao
AU - Yang, Zhaochu
AU - Karlsen, Frank
PY - 2011
Y1 - 2011
N2 - In the present work, the microfabricated impedance sensing array (MISA) unit, based on interdigitated microelectrodes (IDMEs) coated with nickel hexacyanoferrate (NiHCF) film, is presented as an alternative solution to detect pathogens. The NiHCF film, which provide a [Fe(CN)6]3-/4- probe-free redox reaction, was functionalized with hexahistine tags (6His) antibodies with which they have the high-affinity. Variation in the interfacial impedance caused by bio-activated beads was measured to detect targeted Infectious Salmon Anaemia Virus (ISAV) antigen. A detection limit of 10pg/ml was demonstrated, which indicates that the MISA unit is promising to detect pathogens at early-stage of the infection.
AB - In the present work, the microfabricated impedance sensing array (MISA) unit, based on interdigitated microelectrodes (IDMEs) coated with nickel hexacyanoferrate (NiHCF) film, is presented as an alternative solution to detect pathogens. The NiHCF film, which provide a [Fe(CN)6]3-/4- probe-free redox reaction, was functionalized with hexahistine tags (6His) antibodies with which they have the high-affinity. Variation in the interfacial impedance caused by bio-activated beads was measured to detect targeted Infectious Salmon Anaemia Virus (ISAV) antigen. A detection limit of 10pg/ml was demonstrated, which indicates that the MISA unit is promising to detect pathogens at early-stage of the infection.
KW - bio-activated microbeads
KW - interdigitated microelectrodes
KW - interfacial impedance sensor
KW - nickel hexacyanoferrate film
KW - pathogen detection
UR - https://www.scopus.com/pages/publications/80053424159
U2 - 10.1109/EMEIT.2011.6023694
DO - 10.1109/EMEIT.2011.6023694
M3 - 会议稿件
AN - SCOPUS:80053424159
SN - 9781612840857
T3 - Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011
SP - 2841
EP - 2844
BT - Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011
T2 - 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011
Y2 - 12 August 2011 through 14 August 2011
ER -