Derivation of an iPSC line using the Sendai virus from a newborn boy with Alagille Syndrome

Rui Zhou; Pan Liu; Xiaokang Peng; Li Tang; Yanmin Zhang; Xiaoguai Liu

doi:10.1016/j.scr.2026.103927

Derivation of an iPSC line using the Sendai virus from a newborn boy with Alagille Syndrome

Rui Zhou
, Pan Liu
, Xiaokang Peng
, Li Tang
, Yanmin Zhang
, Xiaoguai Liu

医学部

Xi’an Children’s Hospital
Xi'an Jiaotong University

科研成果: 期刊稿件 › 文章 › 同行评审

摘要

We developed a well-characterized induced pluripotent stem cell (iPSC) line. This line was generated by reprogramming peripheral blood mononuclear cells from a male Alagille Syndrome baby using the Sendai viral method, which maintains genomic stability and pluripotent capacity. Markers of undifferentiated iPSCs were validated via immunocytochemistry. Trilineage potential was confirmed through immunofluorescent detection of lineage-specific markers (endoderm, mesoderm, ectoderm). Karyotypic analysis verified a normal diploid complement (46, XY). Additional assessments demonstrated Sendai virus clearance and absence of mycoplasma contamination. This iPSC line serves as a robust resource for disease modeling and regenerative medicine research.

源语言	英语
文章编号	103927
期刊	Stem Cell Research
卷	92
DOI	https://doi.org/10.1016/j.scr.2026.103927
出版状态	已出版 - 4月 2026

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abstract = "We developed a well-characterized induced pluripotent stem cell (iPSC) line. This line was generated by reprogramming peripheral blood mononuclear cells from a male Alagille Syndrome baby using the Sendai viral method, which maintains genomic stability and pluripotent capacity. Markers of undifferentiated iPSCs were validated via immunocytochemistry. Trilineage potential was confirmed through immunofluorescent detection of lineage-specific markers (endoderm, mesoderm, ectoderm). Karyotypic analysis verified a normal diploid complement (46, XY). Additional assessments demonstrated Sendai virus clearance and absence of mycoplasma contamination. This iPSC line serves as a robust resource for disease modeling and regenerative medicine research.",

author = "Rui Zhou and Pan Liu and Xiaokang Peng and Li Tang and Yanmin Zhang and Xiaoguai Liu",

note = "Publisher Copyright: {\textcopyright} 2026 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license. http://creativecommons.org/licenses/by/4.0/",

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AU - Zhou, Rui

AU - Liu, Pan

AU - Peng, Xiaokang

AU - Tang, Li

AU - Zhang, Yanmin

AU - Liu, Xiaoguai

PY - 2026/4

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N2 - We developed a well-characterized induced pluripotent stem cell (iPSC) line. This line was generated by reprogramming peripheral blood mononuclear cells from a male Alagille Syndrome baby using the Sendai viral method, which maintains genomic stability and pluripotent capacity. Markers of undifferentiated iPSCs were validated via immunocytochemistry. Trilineage potential was confirmed through immunofluorescent detection of lineage-specific markers (endoderm, mesoderm, ectoderm). Karyotypic analysis verified a normal diploid complement (46, XY). Additional assessments demonstrated Sendai virus clearance and absence of mycoplasma contamination. This iPSC line serves as a robust resource for disease modeling and regenerative medicine research.

AB - We developed a well-characterized induced pluripotent stem cell (iPSC) line. This line was generated by reprogramming peripheral blood mononuclear cells from a male Alagille Syndrome baby using the Sendai viral method, which maintains genomic stability and pluripotent capacity. Markers of undifferentiated iPSCs were validated via immunocytochemistry. Trilineage potential was confirmed through immunofluorescent detection of lineage-specific markers (endoderm, mesoderm, ectoderm). Karyotypic analysis verified a normal diploid complement (46, XY). Additional assessments demonstrated Sendai virus clearance and absence of mycoplasma contamination. This iPSC line serves as a robust resource for disease modeling and regenerative medicine research.

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JO - Stem Cell Research

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Derivation of an iPSC line using the Sendai virus from a newborn boy with Alagille Syndrome

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