TY - JOUR
T1 - Cytotoxicity of titanium dioxide nanoparticles differs in four liver cells from human and rat
AU - Sha, Baoyong
AU - Gao, Wei
AU - Wang, Shuqi
AU - Xu, Feng
AU - Lu, Tianjian
PY - 2011/12
Y1 - 2011/12
N2 - Titanium dioxide (TiO2) nanoparticles (NPs) are the important nanoscale components of composites. Although TiO2 NPs and their related nanocomposites have been widely used in industrial and medical applications, the adverse effects of TiO2 nanomaterials have not been well studied. Here, we investigated the cytotoxicity of TiO2 NPs in vitro using four liver cell lines: human hepatocellular carcinoma cell line (SMMC-7721), human liver cell line (HL-7702), rat hepatocarcinoma cell line (CBRH-7919) and rat liver cell line (BRL-3A). We checked cell viability, cell morphology, and the levels of reactive oxygen species (ROS) and glutathione (GSH) after TiO2 exposure at varying concentrations (0.1-100 μg/mL) and different exposure periods of time (12-48 h). Compared to the NP-free control, all four cell lines exposed to TiO2 NPs showed cytotoxicity in a dosage-dependent and time-dependent manner, which was associated with the changes of cell viability and cell morphology, increased intercellular ROS levels, and decreased intracellular GSH levels. Further, we observed that carcinomatous liver cells and human liver cells exhibited more tolerance to TiO2 NPs exposure for 24 h, compared to normal liver cells and rat liver cells, respectively. The results indicate that the in vitro cytotoxicity induced by NPs should be assessed with great caution before the use of nanocomposites and that there is a need to standardize the cytotoxicity testing procedure of nanoscale components in composites when using different cell lines.
AB - Titanium dioxide (TiO2) nanoparticles (NPs) are the important nanoscale components of composites. Although TiO2 NPs and their related nanocomposites have been widely used in industrial and medical applications, the adverse effects of TiO2 nanomaterials have not been well studied. Here, we investigated the cytotoxicity of TiO2 NPs in vitro using four liver cell lines: human hepatocellular carcinoma cell line (SMMC-7721), human liver cell line (HL-7702), rat hepatocarcinoma cell line (CBRH-7919) and rat liver cell line (BRL-3A). We checked cell viability, cell morphology, and the levels of reactive oxygen species (ROS) and glutathione (GSH) after TiO2 exposure at varying concentrations (0.1-100 μg/mL) and different exposure periods of time (12-48 h). Compared to the NP-free control, all four cell lines exposed to TiO2 NPs showed cytotoxicity in a dosage-dependent and time-dependent manner, which was associated with the changes of cell viability and cell morphology, increased intercellular ROS levels, and decreased intracellular GSH levels. Further, we observed that carcinomatous liver cells and human liver cells exhibited more tolerance to TiO2 NPs exposure for 24 h, compared to normal liver cells and rat liver cells, respectively. The results indicate that the in vitro cytotoxicity induced by NPs should be assessed with great caution before the use of nanocomposites and that there is a need to standardize the cytotoxicity testing procedure of nanoscale components in composites when using different cell lines.
KW - Cytotoxicity
KW - D. Electron microscopy
KW - D. Optical microscopy
UR - https://www.scopus.com/pages/publications/80053285208
U2 - 10.1016/j.compositesb.2011.05.009
DO - 10.1016/j.compositesb.2011.05.009
M3 - 文章
AN - SCOPUS:80053285208
SN - 1359-8368
VL - 42
SP - 2136
EP - 2144
JO - Composites Part B: Engineering
JF - Composites Part B: Engineering
IS - 8
ER -