Controlled asymmetrical differentiation of Mouse embryoid bodies in microwells with designed heterogeneous biochemical features

We report a novel engineered microwells to spatially control differentiation of mouse embryoid bodies. With integrating multiple functionally distinct biomaterials by soft-photolithography technology, this method enables simple and reliable manufacture of biochemically heterogeneous microwells that are capable of regulating differentiation of stem cell in a spatial-specific manner. This simple technology offers a new dimension of spatial control over embryoid bodies development and has great potential in tissue engineering and biomedical applications.