Construction and identification of Ksp-cadherin-Gpx1-Klk1 expression vector

OBJECTIVE: To construct a Gpx1 and klk1 recombinant vector containing the kidney-specific promoter Ksp-cadherin. METHODS: Human Gpx1, Klk1 and Ksp-cadherin cDNAs were amplified with PCR and inserted in a stepwise manner into the expressive vector pIRES-EGFP to construct the recombinant vector Ksp-cadherin-Gpx1-Klk1. The constructed vector was verified with restriction enzyme digestion and sequence analysis. RESULTS AND CONCLUSION: The recombinant expression vector Ksp-cadherin-Gpx1-Klk1 was constructed and identified successfully, which provides a potent tool for preparing transgenic animals to investigate gene therapy for ischemia-reperfusion injury in kidney transplantation.