TY - JOUR
T1 - Construct a Magnetic Pt/Ru Alloy Peroxidase Mimic As a Reusable and Cost-Effective “Signal-Off” Sensing Platform for Sensitive and Wide-Linear-Range Assay
AU - Gao, Xiangcheng
AU - Liu, Lan
AU - Hou, Haiwei
AU - Jia, Weijuan
AU - Zhang, Aoxue
AU - Zhang, Bo
AU - Bu, Yazhong
AU - Gong, Yining
AU - Yan, Liang
AU - Du, Baoji
N1 - Publisher Copyright:
© 2024 American Chemical Society.
PY - 2024/6/25
Y1 - 2024/6/25
N2 - “Signal-off” nanozyme sensing platforms are usually employed to detect analytes (e.g., ascorbic acid (AA) and alkaline phosphatase (ALP)), which are mostly based on oxidase (OXD) nanozymes. However, their drawbacks, like dissolved oxygen-dependent catalysis capability, relatively low enzyme activity, limited amount, and kind, may not favor sensing platforms’ optimization. Meanwhile, with the need for sustainable development, a reusable “signal-off” sensing platform is essential for cutting down the cost of the assay, but it is rarely developed in previous studies. Magnetic peroxidase (POD) nanozymes potentially make up the deficiencies and become reusable and better “signal-off” sensing platforms. As a proof of concept, we first construct Fe3O4@polydopamine-supported Pt/Ru alloy nanoparticles (IOP@Pt/Ru) without stabilizers. IOP@Pt/Ru shows high POD activity with Vmax of 83.24 × 10-8 M·s-1 for 3,3′,5,5′-Tetramethylbenzidine (TMB) oxidation. Meanwhile, its oxidation rate for TMB is slower than the reduction of oxidized TMB by reducers, favorable for a more significant detection signal. On the other hand, IOP@Pt/Ru possesses great magnet-responsive capability, making itself be recycled and reused for at least 15-round catalysis. When applying IOP@Pt/Ru for AA (ALP) detection, it performs better detectable adaptability, with a linear range of 0.01-0.2 mM (0.1-100 U/L) and a limit of detection of 0.01 mM (0.05 U/L), superior to most of OXD nanozyme-based ALP sensing platform. Finally, IOP@Pt/Ru’s reusable assay was demonstrated in real blood samples for ALP assay, which has never been explored in previous studies. Overall, this study develops a reusable “signal-off” nanozyme sensing platform with superior assay capabilities than traditional OXD nanozymes, paves a new way to optimize nanozyme-based “signal-off” sensing platforms, and provides an idea for constructing inexpensive and sustainable sensing platforms.
AB - “Signal-off” nanozyme sensing platforms are usually employed to detect analytes (e.g., ascorbic acid (AA) and alkaline phosphatase (ALP)), which are mostly based on oxidase (OXD) nanozymes. However, their drawbacks, like dissolved oxygen-dependent catalysis capability, relatively low enzyme activity, limited amount, and kind, may not favor sensing platforms’ optimization. Meanwhile, with the need for sustainable development, a reusable “signal-off” sensing platform is essential for cutting down the cost of the assay, but it is rarely developed in previous studies. Magnetic peroxidase (POD) nanozymes potentially make up the deficiencies and become reusable and better “signal-off” sensing platforms. As a proof of concept, we first construct Fe3O4@polydopamine-supported Pt/Ru alloy nanoparticles (IOP@Pt/Ru) without stabilizers. IOP@Pt/Ru shows high POD activity with Vmax of 83.24 × 10-8 M·s-1 for 3,3′,5,5′-Tetramethylbenzidine (TMB) oxidation. Meanwhile, its oxidation rate for TMB is slower than the reduction of oxidized TMB by reducers, favorable for a more significant detection signal. On the other hand, IOP@Pt/Ru possesses great magnet-responsive capability, making itself be recycled and reused for at least 15-round catalysis. When applying IOP@Pt/Ru for AA (ALP) detection, it performs better detectable adaptability, with a linear range of 0.01-0.2 mM (0.1-100 U/L) and a limit of detection of 0.01 mM (0.05 U/L), superior to most of OXD nanozyme-based ALP sensing platform. Finally, IOP@Pt/Ru’s reusable assay was demonstrated in real blood samples for ALP assay, which has never been explored in previous studies. Overall, this study develops a reusable “signal-off” nanozyme sensing platform with superior assay capabilities than traditional OXD nanozymes, paves a new way to optimize nanozyme-based “signal-off” sensing platforms, and provides an idea for constructing inexpensive and sustainable sensing platforms.
UR - https://www.scopus.com/pages/publications/85196041652
U2 - 10.1021/acs.analchem.4c02156
DO - 10.1021/acs.analchem.4c02156
M3 - 文章
AN - SCOPUS:85196041652
SN - 0003-2700
VL - 96
SP - 10467
EP - 10475
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 25
ER -