TY - JOUR
T1 - Better quality and more usable embryos obtained on day 3 cultured in 5% than 20% oxygen
T2 - A controlled and randomized study using the sibling oocytes
AU - Peng, Huixia
AU - Shi, Wenhao
AU - Zhang, Wei
AU - Xue, Xia
AU - Li, Na
AU - Li, Wei
AU - Shi, Juanzi
N1 - Publisher Copyright:
© Society for Gynecologic Investigation.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - To evaluate the effect of atmospheric oxygen (O2) concentration on embryonic development, a controlled and randomized study using the sibling oocytes was carried out. A total of 147 patients were studied. Embryos were cultured in O2 concentration 20% versus 5% during the gamete, zygote, and first 3 days. The mean cell numbers of embryo (7.69 ± 1.91 vs 7.20 ± 1.82, P =.011) and rate of clinically useable embryos (81.62% vs 77.22%, P =.017) were significantly higher in 5% O2 than in 20% O2. There was no difference in the zygote developmental stage, day 2, day 4, and blastocyst stage. The quality of blastocyst (both inner cell mass and trophectoderm) showed no difference. Also, there was no increase in embryos fragmentation and uneven cells in 20% O2 culture condition. In conclusion, 20% O2 reduced the mean cell numbers of embryo and the number of clinically useable embryos on day 3. However, there was no subsequent negative impact on development of day 4 and blastocyst stage.
AB - To evaluate the effect of atmospheric oxygen (O2) concentration on embryonic development, a controlled and randomized study using the sibling oocytes was carried out. A total of 147 patients were studied. Embryos were cultured in O2 concentration 20% versus 5% during the gamete, zygote, and first 3 days. The mean cell numbers of embryo (7.69 ± 1.91 vs 7.20 ± 1.82, P =.011) and rate of clinically useable embryos (81.62% vs 77.22%, P =.017) were significantly higher in 5% O2 than in 20% O2. There was no difference in the zygote developmental stage, day 2, day 4, and blastocyst stage. The quality of blastocyst (both inner cell mass and trophectoderm) showed no difference. Also, there was no increase in embryos fragmentation and uneven cells in 20% O2 culture condition. In conclusion, 20% O2 reduced the mean cell numbers of embryo and the number of clinically useable embryos on day 3. However, there was no subsequent negative impact on development of day 4 and blastocyst stage.
KW - culture condition
KW - embryonic development
KW - low oxygen culture
KW - oxygen concentration
UR - https://www.scopus.com/pages/publications/84957990328
U2 - 10.1177/1933719115602761
DO - 10.1177/1933719115602761
M3 - 文章
C2 - 26342053
AN - SCOPUS:84957990328
SN - 1933-7191
VL - 23
SP - 372
EP - 378
JO - Reproductive Sciences
JF - Reproductive Sciences
IS - 3
ER -