Association between IL-1B + 3954/Taq I gene polymorphisms and chronic periodontitis

Objective: To study whether specific IL-1B + 3954/Taq I genotype alleles are associated with chronic periodontitis (CP) in Chinese of the Han nationality. Methods: CP group consisted of 66 patients while healthy controls group consisted of 50 subjects. Anti-coagulated peripheral blood sample was obtained from each subject and genomic DNA was extracted from each sample. Single nucleotide polymorphisms (SNPs) at IL-1B + 3954/Taq I were analyzed by standard polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) assay. Results: In the control group, higher numerical values of the A1/A1 genotype were observed for the IL-1B + 3954/Taq I SNPs (84.0%), and the A ²⁺ genotype was present in 16% of this sample. Statistical analysis revealed no significant difference between the two groups. Conlusion: There is no evidence in our study supporting the association between single nucleotide polymorphisms (SNPs) of the IL-1B + 3954/Taq I gene and prevalence and/or severity of chronic periodontitis.