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An integrated paper-based microfluidic platform for autonomous, point-of-care ELISA

  • Hao Fu
  • , Qiyang Wu
  • , Chen Zhao
  • , Xiao Li
  • , Nicole Li-Jessen
  • , Xinyu Liu

科研成果: 书/报告/会议事项章节会议稿件同行评审

1 引用 (Scopus)

摘要

This paper describes the first portable paper-based microfluidic platform for autonomous enzymelinked immunosorbent assay (ELISA). Different from previous devices that require repeated reagent pipetting, our paper device integrates shape memory polymer (SMP) based micro-valves for automatically regulating flows and transferring on-chip-stored reagents in a programmed manner. A user simply needs to add sample and buffer solutions to the device, press a button to start the assay, and finally read the result from a screen after 60 min. We perform autonomous ELISAs of rabbit IgG with a limit of detection (LOD) of 27 pM, and demonstrate accurate testing of TNF-a protein in rate samples.

源语言英语
主期刊名20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
出版商Chemical and Biological Microsystems Society
192-193
页数2
ISBN(电子版)9780979806490
出版状态已出版 - 2016
已对外发布
活动20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, 爱尔兰
期限: 9 10月 201613 10月 2016

出版系列

姓名20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

会议

会议20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
国家/地区爱尔兰
Dublin
时期9/10/1613/10/16

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