TY - JOUR
T1 - Alterations of IL-1beta and TNF-alpha expression in RAW264.7 cell damage induced by two samples of PM2.5 with different compositions
AU - Lyu, Yizhen
AU - Zhou, Jieting
AU - Li, Jianjun
AU - Li, Jin
AU - Hu, Guocheng
AU - Wang, Liyun
AU - Wang, Liang
AU - Han, Jing
AU - Wang, Dong
N1 - Publisher Copyright:
© The Author(s) 2022.
PY - 2022/7
Y1 - 2022/7
N2 - Fine particulate matter 2.5 (PM2.5) has been demonstrated by previous studies to be associated with cell damage. To explore the impact of the composition of PM2.5 on PM2.5-mediated inflammation, this study investigated the composition of PM2.5 collected during the wintertime indoor heating season and observed its inflammatory effect. Samples were collected during the heating season from December 5, 2017, to January 8, 2018, in Xi’an. Compositions of organic carbon (OC), elemental carbon (EC), and water-soluble ions were analysed. Two representative samples (sample 1 and 2) were selected with significant differences in compositions. They were configured into four concentrations (0.1 μg/mL, 1 μg/mL, 10 μg/mL, 20 μg/mL) and used as interventions on RAW264.7 cells for 4 h and 24 h separately. Cell viability was detected by CCK-8. Tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) gene and protein expression levels were detected by real-time quantitative real-time polymerase chain reaction (RT-qPCR) and western blotting. The results showed that the cell viability of sample 1 intervened cells at 4 h and 24 h was lower than that of sample 2. IL-1β gene in most PM2.5 intervention groups was lower than in the control group. Protein expression was higher at 4 h than at 24 h. In conclusion, PM2.5 components influence cell viability and expression of IL-1β and TNF-α, while high concentrations of NO3−, Cl−, Na+, K+, Mg2+, Ca2+, and others in the PM2.5 composition have a significant harmful effect.
AB - Fine particulate matter 2.5 (PM2.5) has been demonstrated by previous studies to be associated with cell damage. To explore the impact of the composition of PM2.5 on PM2.5-mediated inflammation, this study investigated the composition of PM2.5 collected during the wintertime indoor heating season and observed its inflammatory effect. Samples were collected during the heating season from December 5, 2017, to January 8, 2018, in Xi’an. Compositions of organic carbon (OC), elemental carbon (EC), and water-soluble ions were analysed. Two representative samples (sample 1 and 2) were selected with significant differences in compositions. They were configured into four concentrations (0.1 μg/mL, 1 μg/mL, 10 μg/mL, 20 μg/mL) and used as interventions on RAW264.7 cells for 4 h and 24 h separately. Cell viability was detected by CCK-8. Tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) gene and protein expression levels were detected by real-time quantitative real-time polymerase chain reaction (RT-qPCR) and western blotting. The results showed that the cell viability of sample 1 intervened cells at 4 h and 24 h was lower than that of sample 2. IL-1β gene in most PM2.5 intervention groups was lower than in the control group. Protein expression was higher at 4 h than at 24 h. In conclusion, PM2.5 components influence cell viability and expression of IL-1β and TNF-α, while high concentrations of NO3−, Cl−, Na+, K+, Mg2+, Ca2+, and others in the PM2.5 composition have a significant harmful effect.
KW - IL-1β
KW - PM
KW - TNF-α
KW - macrophage
UR - https://www.scopus.com/pages/publications/85133986308
U2 - 10.1177/00368504221113709
DO - 10.1177/00368504221113709
M3 - 文章
C2 - 35833342
AN - SCOPUS:85133986308
SN - 0036-8504
VL - 105
JO - Science Progress
JF - Science Progress
IS - 3
ER -