[Acyldepsipeptide 1 induces apoptosis in renal cancer cells by down-regulation of Gli and Bcl-2 via SHH pathway].

To investigate the effects of acyldepsipeptide 1 (ADEP1) on renal cancer cell apoptosis and on the expressions of SHH signaling pathway related protein Gli-1 and Bcl-2. The renal cancer cells (ACHN and 769-P) were treated with 50 μmol/L ADEP1 for 48 hours. The changes of cell morphology and the nucleus shape in cell apoptosis were observed under a light microscope, and cell viability was measured by MTT assay. The mRNA levels of SHH and Gli-1 were detected by real-time quantitative PCR (qRT-PCR), and the protein levels of Gli-1 and Bcl-2 were determined by Western blotting. ADEP1 induced renal cancer cell apoptosis through reducing the transcription of SHH and Gli-1, and decreasing Gli-1 and Bcl-2 protein expressions. ADEP1 could induce renal cancer cell apoptosis by the inhibition on SHH signaling pathway related proteins.