A conjugated polymer fluorescent sensor for continuous identification of copper(II) and pyrophosphate in blood serum and synovial fluid

A novel "on-off-on" super-sensitive conjugated polymer fluorescence sensor (PPE-DPA) was developed and it was applied to realize the continuous recognition of Cu²⁺ and pyrophosphate (PPi). The fluorescence intensity decreased linearly with the change of Cu²⁺ from 0.05 to 5.0 μmol L^-1 and the limit of detection was 24 nmol L^-1. The fluorescence intensity was linearly enhanced with the increase of PPi from 0.5 to 12.0 μmol L^-1 and the limit of detection was 230 nmol L^-1. In addition, this method was applied to detect PPi in the blood serum and synovial fluid of patients with arthritis and satisfactory results were obtained. Thus, the PPE-DPA is not only an effective tool for detecting Cu²⁺ and PPi in samples, but also presents a potential way to diagnose arthritis.