等离子体活化水在糠秕马拉色菌灭活中的应用

To address the limitations of existing methods for inactivating Malassezia furfur, such as poor universality, low compliance, adverse reactions, and tendency to induce drug resistance, the application of plasma-activated water (PAW) for its inactivation is explored. Different types of PAW are prepared using low-tempe rature plasma technology, and the variations in their physicochemical properties are investigated. Changes in the viability of Malassezia furfur before and after inactivation are analyzed based on the colony counting method. The inactivation mechanism is analyzed by detecting DNA and protein leakage? extracellular alkaline phosphatase (AKP) activity, and the content of UV-absorbing substances. The results indicate that PAW contains a large number of reactive species, including H⁺, H₂ O₂, NO⁻ ₂, NO⁻₃, etc. Significant inactivation of Malassezia furfur is achieved when the gas flow rate is 5 L • min⁻¹ and the activation time is 25 min, reducing the colony count to 100.98 CFU • mL⁻]. The inactivation leads to increased leakage of DNA and proteins, as well as elevated levels of extracellular AKP and UV-absorbing substances. This study provides theoretical support for the application of PAW in preventing and controlling Malassezia furfur.