白藜芦醇通过调节Hippo信号通路抑制胰腺星状细胞活化

Objective： To investigate the inhibitory effect of resveratrol on the activation of pancreatic stellate cells and to explore the role of the Hippo signaling pathway in it. Methods： Pancreatic stellate cells (PSCs) were isolated by trypsin digestion and then treated with resveratrol. Western blot and immunofluorescence staining were used to detect the activation of the cells. Transwell invasion assay and Western blot were used to detect the invasive ability of Panc-1 and the expressions of E-cadherin and Vimentin in pancreatic cancer cells (PCs)-PSCs indirect co-culture conditions. Results： Resveratrol could inhibit the activation of PSCs and inhibit YAP, CTGF and CYR61 expressions. After co-culture with PSCs condition medium pre-treated with resveratrol, the invasive ability of Panc-1 cells was significant reduced (P<0.05). E-cadherin expression was increased while Vimentin expression was decreased (P<0.05). Conclusion： Resveratrol can inhibit the activation of pancreatic stellate cells via inhibiting YAP expression and can also inhibit the invasion and EMT transformation of Panc-1 cells under co-culture conditions.