常压低温等离子体对羊肚菌培养基质的杀菌效果及工艺优化

Morel culture substrate was sterilized by plasma technology at normal pressure and low temperature. The effects of four single factor variables, voltage, frequency, treatment time and treatment times, on colony number and water content were investigated, and the treatment conditions were optimized by orthogonal test. The results showed that the sterilization rate of bacteria and fungi increased with the increase of voltage, time and times of atmospheric plasma treatment, and the water loss also increased. With the increase of plasma treatment frequency, the sterilization rate first increased and then decreased, showing a tendency of “volcanic type”. There were significant differences in germicidal effect under different treatment conditions (P<0.05). The orthogonal test showed that the influencing degree of the four factors was ranged as frequency>voltage>treatment time>treatment times. The optimal sterilization condition was as follows: voltage 30 kV, frequency 9.6 kHz, time 45 min, once a treatment. Under this condition, the sterilization rates of bacteria and fungi were 96.87% and 93.77%, respectively, and the water loss was less.