Visual detection of HPV16 using a photoactivatable CRISPR-Cas12 system

Xiaoya Gu; Zhe Ma; Lin Zhou; Na Li; Shijiang Yu; Fu Wang; Ruifang An

doi:10.1039/d5cc00369e

Visual detection of HPV16 using a photoactivatable CRISPR-Cas12 system

Xiaoya Gu
, Zhe Ma
, Lin Zhou
, Na Li
, Shijiang Yu
, Fu Wang
, Ruifang An

Health Science Center

The First Affiliated Hospital of Xi’an Jiaotong University
Xi'an Jiaotong University
The Second Affiliated Hospital of Xi'an Jiaotong University

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Human papillomavirus (HPV) screening is crucial for the diagnosis of cervical cancer. In this study, we have combined photoactivated CRISPR-Cas12a with tube-in-tube structure and recombinase polymerase amplification (RPA) to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm. It serves as a potential tool for on-site diagnostic use, which could be beneficial in terms of portability and speed.

Original language	English
Pages (from-to)	4383-4386
Number of pages	4
Journal	Chemical Communications
Volume	61
Issue number	22
DOIs	https://doi.org/10.1039/d5cc00369e
State	Published - 18 Feb 2025

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1039/d5cc00369e

Cite this

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abstract = "Human papillomavirus (HPV) screening is crucial for the diagnosis of cervical cancer. In this study, we have combined photoactivated CRISPR-Cas12a with tube-in-tube structure and recombinase polymerase amplification (RPA) to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm. It serves as a potential tool for on-site diagnostic use, which could be beneficial in terms of portability and speed.",

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AU - Gu, Xiaoya

AU - Ma, Zhe

AU - Zhou, Lin

AU - Li, Na

AU - Yu, Shijiang

AU - Wang, Fu

AU - An, Ruifang

PY - 2025/2/18

Y1 - 2025/2/18

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AB - Human papillomavirus (HPV) screening is crucial for the diagnosis of cervical cancer. In this study, we have combined photoactivated CRISPR-Cas12a with tube-in-tube structure and recombinase polymerase amplification (RPA) to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm. It serves as a potential tool for on-site diagnostic use, which could be beneficial in terms of portability and speed.

UR - https://www.scopus.com/pages/publications/85219075321

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DO - 10.1039/d5cc00369e

M3 - 文章

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JO - Chemical Communications

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ER -

Visual detection of HPV16 using a photoactivatable CRISPR-Cas12 system

Abstract

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