Validated liquid chromatography mass spectrometry method for quantitative determination of strictosamide in dog plasma and its application to pharmacokinetic study

Chen Dai; Wei Xiao; Yan Liang; Lin Xie; Guangji Wang; Gang Ding; Zhaoqing Meng; Juan Zhang; Tianye Guan; An Kang; Xiao Zheng; Tong Xie; Chunzhu Li; Qijin Zhao; Wenjun Liu; Li Zhao; Jia Xu

doi:10.1002/bmc.1606

Validated liquid chromatography mass spectrometry method for quantitative determination of strictosamide in dog plasma and its application to pharmacokinetic study

Chen Dai
, Wei Xiao
, Yan Liang
, Lin Xie
, Guangji Wang
, Gang Ding
, Zhaoqing Meng
, Juan Zhang
, Tianye Guan
, An Kang
, Xiao Zheng
, Tong Xie
, Chunzhu Li
, Qijin Zhao
, Wenjun Liu
, Li Zhao
, Jia Xu

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A simple, sensitive and specific high-performance liquid chromatography mass spectrometry (LC-MS) method was developed and validated for the quantification of strictosamide in dog plasma. Strictosamide and internal standard (IS, ranolazine) extracted by liquid-liquid extraction with ethyl acetate were separated on a C₁₈ column using a gradient elution program. The detection was performed by selected ion monitoring mode via a positive electrospray ionization interface. The LLOQ was 1.0ng/mL and the method exhibited acceptable precision, extraction efficiency and matrix effect. Finally, this proposed method was successfully applied to dog pharmacokinetic study and yielded the most comprehensive data on systemic exposure of strictosamide to date.

Original language	English
Pages (from-to)	1338-1342
Number of pages	5
Journal	Biomedical Chromatography
Volume	25
Issue number	12
DOIs	https://doi.org/10.1002/bmc.1606
State	Published - Dec 2011
Externally published	Yes

Keywords

LC-MS
Pharmacokinetic study
Strictosamide

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10.1002/bmc.1606

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Dai, C., Xiao, W., Liang, Y., Xie, L., Wang, G., Ding, G., Meng, Z., Zhang, J., Guan, T., Kang, A., Zheng, X., Xie, T., Li, C., Zhao, Q., Liu, W., Zhao, L., & Xu, J. (2011). Validated liquid chromatography mass spectrometry method for quantitative determination of strictosamide in dog plasma and its application to pharmacokinetic study. Biomedical Chromatography, 25(12), 1338-1342. https://doi.org/10.1002/bmc.1606

@article{ab959d1291314e159f2cd8c88115665e,

title = "Validated liquid chromatography mass spectrometry method for quantitative determination of strictosamide in dog plasma and its application to pharmacokinetic study",

abstract = "A simple, sensitive and specific high-performance liquid chromatography mass spectrometry (LC-MS) method was developed and validated for the quantification of strictosamide in dog plasma. Strictosamide and internal standard (IS, ranolazine) extracted by liquid-liquid extraction with ethyl acetate were separated on a C18 column using a gradient elution program. The detection was performed by selected ion monitoring mode via a positive electrospray ionization interface. The LLOQ was 1.0ng/mL and the method exhibited acceptable precision, extraction efficiency and matrix effect. Finally, this proposed method was successfully applied to dog pharmacokinetic study and yielded the most comprehensive data on systemic exposure of strictosamide to date.",

keywords = "LC-MS, Pharmacokinetic study, Strictosamide",

author = "Chen Dai and Wei Xiao and Yan Liang and Lin Xie and Guangji Wang and Gang Ding and Zhaoqing Meng and Juan Zhang and Tianye Guan and An Kang and Xiao Zheng and Tong Xie and Chunzhu Li and Qijin Zhao and Wenjun Liu and Li Zhao and Jia Xu",

year = "2011",

month = dec,

doi = "10.1002/bmc.1606",

language = "英语",

volume = "25",

pages = "1338--1342",

journal = "Biomedical Chromatography",

issn = "0269-3879",

publisher = "John Wiley and Sons Ltd",

number = "12",

}

Dai, C, Xiao, W, Liang, Y, Xie, L, Wang, G, Ding, G, Meng, Z, Zhang, J, Guan, T, Kang, A, Zheng, X, Xie, T, Li, C, Zhao, Q, Liu, W, Zhao, L & Xu, J 2011, 'Validated liquid chromatography mass spectrometry method for quantitative determination of strictosamide in dog plasma and its application to pharmacokinetic study', Biomedical Chromatography, vol. 25, no. 12, pp. 1338-1342. https://doi.org/10.1002/bmc.1606

TY - JOUR

T1 - Validated liquid chromatography mass spectrometry method for quantitative determination of strictosamide in dog plasma and its application to pharmacokinetic study

AU - Dai, Chen

AU - Xiao, Wei

AU - Liang, Yan

AU - Xie, Lin

AU - Wang, Guangji

AU - Ding, Gang

AU - Meng, Zhaoqing

AU - Zhang, Juan

AU - Guan, Tianye

AU - Kang, An

AU - Zheng, Xiao

AU - Xie, Tong

AU - Li, Chunzhu

AU - Zhao, Qijin

AU - Liu, Wenjun

AU - Zhao, Li

AU - Xu, Jia

PY - 2011/12

Y1 - 2011/12

N2 - A simple, sensitive and specific high-performance liquid chromatography mass spectrometry (LC-MS) method was developed and validated for the quantification of strictosamide in dog plasma. Strictosamide and internal standard (IS, ranolazine) extracted by liquid-liquid extraction with ethyl acetate were separated on a C18 column using a gradient elution program. The detection was performed by selected ion monitoring mode via a positive electrospray ionization interface. The LLOQ was 1.0ng/mL and the method exhibited acceptable precision, extraction efficiency and matrix effect. Finally, this proposed method was successfully applied to dog pharmacokinetic study and yielded the most comprehensive data on systemic exposure of strictosamide to date.

AB - A simple, sensitive and specific high-performance liquid chromatography mass spectrometry (LC-MS) method was developed and validated for the quantification of strictosamide in dog plasma. Strictosamide and internal standard (IS, ranolazine) extracted by liquid-liquid extraction with ethyl acetate were separated on a C18 column using a gradient elution program. The detection was performed by selected ion monitoring mode via a positive electrospray ionization interface. The LLOQ was 1.0ng/mL and the method exhibited acceptable precision, extraction efficiency and matrix effect. Finally, this proposed method was successfully applied to dog pharmacokinetic study and yielded the most comprehensive data on systemic exposure of strictosamide to date.

KW - LC-MS

KW - Pharmacokinetic study

KW - Strictosamide

UR - https://www.scopus.com/pages/publications/80055097100

U2 - 10.1002/bmc.1606

DO - 10.1002/bmc.1606

M3 - 文章

C2 - 21387351

AN - SCOPUS:80055097100

SN - 0269-3879

VL - 25

SP - 1338

EP - 1342

JO - Biomedical Chromatography

JF - Biomedical Chromatography

IS - 12

ER -

Validated liquid chromatography mass spectrometry method for quantitative determination of strictosamide in dog plasma and its application to pharmacokinetic study

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