Use of anchorchip-time-of-flight spectrometry technology to screen tumor biomarker proteins in serum for small cell lung cancer

Jie Du; Shuanying Yang; Xiuli Lin; Lina Bu; Yandong Nan; Shufen Huo; Wenli Shang

doi:10.1186/1746-1596-5-60

Use of anchorchip-time-of-flight spectrometry technology to screen tumor biomarker proteins in serum for small cell lung cancer

Jie Du
, Shuanying Yang
, Xiuli Lin
, Lina Bu
, Yandong Nan
, Shufen Huo
, Wenli Shang

Health Science Center

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Background: The purpose of this study is to discover potential biomarkers in serum for the detection of small cell lung cancer (SCLC).Methods: 74 serum samples including 30 from SCLC patients and 44 from healthy controls were analyzed using ClinProt system combined with matrix-assisted laser desorption/ionization time-of-flight masss spectrometry (MALDI-TOF-MS). ClinProt software and genetic algorithm analysis selected a panel of serum markers that most efficiently predicted which patients had SCLC.Results: The diagnostic pattern combined with 5 potential biomarkers could differentiate SCLC patients from healthy persons, with a sensitivity of 90%, specificity of 97.73%. Remarkably, 88.89% of stage I/II patients were accurately assigned to SCLC.Conclusions: Anchorchip-time-of-flight spectrometry technology will provide a highly accurate approach for discovering new biomarkers for the detection of SCLC.

Original language	English
Article number	60
Journal	Diagnostic Pathology
Volume	5
Issue number	1
DOIs	https://doi.org/10.1186/1746-1596-5-60
State	Published - 20 Sep 2010

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title = "Use of anchorchip-time-of-flight spectrometry technology to screen tumor biomarker proteins in serum for small cell lung cancer",

abstract = "Background: The purpose of this study is to discover potential biomarkers in serum for the detection of small cell lung cancer (SCLC).Methods: 74 serum samples including 30 from SCLC patients and 44 from healthy controls were analyzed using ClinProt system combined with matrix-assisted laser desorption/ionization time-of-flight masss spectrometry (MALDI-TOF-MS). ClinProt software and genetic algorithm analysis selected a panel of serum markers that most efficiently predicted which patients had SCLC.Results: The diagnostic pattern combined with 5 potential biomarkers could differentiate SCLC patients from healthy persons, with a sensitivity of 90\%, specificity of 97.73\%. Remarkably, 88.89\% of stage I/II patients were accurately assigned to SCLC.Conclusions: Anchorchip-time-of-flight spectrometry technology will provide a highly accurate approach for discovering new biomarkers for the detection of SCLC.",

author = "Jie Du and Shuanying Yang and Xiuli Lin and Lina Bu and Yandong Nan and Shufen Huo and Wenli Shang",

year = "2010",

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doi = "10.1186/1746-1596-5-60",

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TY - JOUR

T1 - Use of anchorchip-time-of-flight spectrometry technology to screen tumor biomarker proteins in serum for small cell lung cancer

AU - Du, Jie

AU - Yang, Shuanying

AU - Lin, Xiuli

AU - Bu, Lina

AU - Nan, Yandong

AU - Huo, Shufen

AU - Shang, Wenli

PY - 2010/9/20

Y1 - 2010/9/20

N2 - Background: The purpose of this study is to discover potential biomarkers in serum for the detection of small cell lung cancer (SCLC).Methods: 74 serum samples including 30 from SCLC patients and 44 from healthy controls were analyzed using ClinProt system combined with matrix-assisted laser desorption/ionization time-of-flight masss spectrometry (MALDI-TOF-MS). ClinProt software and genetic algorithm analysis selected a panel of serum markers that most efficiently predicted which patients had SCLC.Results: The diagnostic pattern combined with 5 potential biomarkers could differentiate SCLC patients from healthy persons, with a sensitivity of 90%, specificity of 97.73%. Remarkably, 88.89% of stage I/II patients were accurately assigned to SCLC.Conclusions: Anchorchip-time-of-flight spectrometry technology will provide a highly accurate approach for discovering new biomarkers for the detection of SCLC.

AB - Background: The purpose of this study is to discover potential biomarkers in serum for the detection of small cell lung cancer (SCLC).Methods: 74 serum samples including 30 from SCLC patients and 44 from healthy controls were analyzed using ClinProt system combined with matrix-assisted laser desorption/ionization time-of-flight masss spectrometry (MALDI-TOF-MS). ClinProt software and genetic algorithm analysis selected a panel of serum markers that most efficiently predicted which patients had SCLC.Results: The diagnostic pattern combined with 5 potential biomarkers could differentiate SCLC patients from healthy persons, with a sensitivity of 90%, specificity of 97.73%. Remarkably, 88.89% of stage I/II patients were accurately assigned to SCLC.Conclusions: Anchorchip-time-of-flight spectrometry technology will provide a highly accurate approach for discovering new biomarkers for the detection of SCLC.

UR - https://www.scopus.com/pages/publications/77956633075

U2 - 10.1186/1746-1596-5-60

DO - 10.1186/1746-1596-5-60

M3 - 文章

C2 - 20854674

AN - SCOPUS:77956633075

SN - 1746-1596

VL - 5

JO - Diagnostic Pathology

JF - Diagnostic Pathology

IS - 1

M1 - 60

ER -

Use of anchorchip-time-of-flight spectrometry technology to screen tumor biomarker proteins in serum for small cell lung cancer

Abstract

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