Ultrasensitive and selective detection of nicotinamide adenine dinucleotide by target-triggered ligation-rolling circle amplification

Yongxi Zhao; Lin Qi; Feng Chen; Yanhua Dong; Yu Kong; Yayan Wu; Chunhai Fan

doi:10.1039/c2cc17422g

Ultrasensitive and selective detection of nicotinamide adenine dinucleotide by target-triggered ligation-rolling circle amplification

Yongxi Zhao
, Lin Qi
, Feng Chen
, Yanhua Dong
, Yu Kong
, Yayan Wu
, Chunhai Fan

School of Life Science and Technology (SLST)

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

An ultrasensitive fluorescence assay for nicotinamide adenine dinucleotide (NAD⁺) was developed by target-triggered ligation-rolling circle amplification (L-RCA). This novel approach can detect as low as 1 pM NAD⁺, much lower than those of previously reported biosensors, and exhibits high discrimination ability even against 200 times excess of NAD⁺ analogs.

Original language	English
Pages (from-to)	3354-3356
Number of pages	3
Journal	Chemical Communications
Volume	48
Issue number	27
DOIs	https://doi.org/10.1039/c2cc17422g
State	Published - 5 Mar 2012

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10.1039/c2cc17422g

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title = "Ultrasensitive and selective detection of nicotinamide adenine dinucleotide by target-triggered ligation-rolling circle amplification",

abstract = "An ultrasensitive fluorescence assay for nicotinamide adenine dinucleotide (NAD+) was developed by target-triggered ligation-rolling circle amplification (L-RCA). This novel approach can detect as low as 1 pM NAD+, much lower than those of previously reported biosensors, and exhibits high discrimination ability even against 200 times excess of NAD+ analogs.",

author = "Yongxi Zhao and Lin Qi and Feng Chen and Yanhua Dong and Yu Kong and Yayan Wu and Chunhai Fan",

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doi = "10.1039/c2cc17422g",

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T1 - Ultrasensitive and selective detection of nicotinamide adenine dinucleotide by target-triggered ligation-rolling circle amplification

AU - Zhao, Yongxi

AU - Qi, Lin

AU - Chen, Feng

AU - Dong, Yanhua

AU - Kong, Yu

AU - Wu, Yayan

AU - Fan, Chunhai

PY - 2012/3/5

Y1 - 2012/3/5

N2 - An ultrasensitive fluorescence assay for nicotinamide adenine dinucleotide (NAD+) was developed by target-triggered ligation-rolling circle amplification (L-RCA). This novel approach can detect as low as 1 pM NAD+, much lower than those of previously reported biosensors, and exhibits high discrimination ability even against 200 times excess of NAD+ analogs.

AB - An ultrasensitive fluorescence assay for nicotinamide adenine dinucleotide (NAD+) was developed by target-triggered ligation-rolling circle amplification (L-RCA). This novel approach can detect as low as 1 pM NAD+, much lower than those of previously reported biosensors, and exhibits high discrimination ability even against 200 times excess of NAD+ analogs.

UR - https://www.scopus.com/pages/publications/84863284791

U2 - 10.1039/c2cc17422g

DO - 10.1039/c2cc17422g

M3 - 文章

C2 - 22361740

AN - SCOPUS:84863284791

SN - 1359-7345

VL - 48

SP - 3354

EP - 3356

JO - Chemical Communications

JF - Chemical Communications

IS - 27

ER -

Ultrasensitive and selective detection of nicotinamide adenine dinucleotide by target-triggered ligation-rolling circle amplification

Abstract

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