Transient Thermal Response of Blood Vessels during Laser Irradiation Monitored by Laser Speckle Contrast Imaging

Real-time monitoring of blood flow and thrombosis formation induced by laser irradiation is critical to reveal the thermal-damage mechanism and successfully implement vascular-dermatology laser surgery. Laser speckle contrast imaging (LSCI) is a non-invasive technique to visualize perfusion in various tissues. However, the ability of the LSCI to monitor the transient thermal response of blood vessels, especially thrombus formation during laser irradiation, requires further research. In this paper, an LSCI system was constructed and a 632 nm He-Ne laser was employed to illuminate a Sprague Dawley rat dorsal skin chamber model irradiated by a 1064 nm Nd: YAG therapy laser. The anisotropic diffusion filtering (ADF) technique is implemented after temporal LSCI (tLSCI) processing to improve the SNR and temporal resolution. The speckle flow index is used to characterize the blood-flow velocity to reduce the computational cost. The combination of the tLSCI and ADF increases the temporal resolution by five times and the SNR by 17.2 times and 16.14 times, without and with laser therapy, respectively. The laser-induced thrombus formation and vascular damage during laser surgery can be visualized without any exogenous labels, which provides a powerful tool for thrombus monitoring during laser surgery.