TNF-α通过Wnt/β-catenin信号通路促进结肠癌细胞的增殖

Objective: To investigate the effect of treatment with TNF-α on growth ability of colon cancer cells and its possible mechanism. Methods: Human colon cancer HT-29 cells were treated with TNF-α (10 nmol/L). The effect of TNF-α on the proliferative ability was examined by cell growth curve assay and MTT assay, respectively. Cell cycle was detected by flow cytometry. Protein levels of β-catenin, c-myc and cyclinD1 were detected by Western blot. We also observed the effect of the Wnt/β-catenin pathway blockage by XAV939 on TNF-α's promoting proliferation of colon cancer cells and proteins related to the Wnt/β-catenin pathway. Results: Upon treatment with TNF-α, the proliferative ability of HT-29 cells was enhanced (all P<0.05), G0/G1 phase cell ratio was decreased (P<0.05), and S phase cell ratio was increased (P<0.05). The protein levels of β-catenin, c-myc and cyclinD1 were increased in TNF-α-treated HT-29 cells (all P<0.05). XAV939 treatment resulted in a significant inhibition of cell proliferation ability (all P<0.05) as well as the protein levels of β-catenin, c-myc and cyclinD1 (all P<0.05) in the TNF-α-treated HT29 cells. Conclusion: TNF-α may be involved in the occurrence and development of colon cancer by activating the Wnt/β-catenin pathway and promoting the proliferation of colon cancer cells.