The role of Th1/Th2 cytokines played in regulation of specific CD4 ⁺ Th1 cell conversion and activation during inflammatory reaction of chronic obstructive pulmonary disease

CD4 ⁺ Th1-CXCR3 signalling pathway may play a key role in chronic obstructive pulmonary disease (COPD). The aim of this study was to explore Th1/Th2 cytokines ratio differences in patients in different stages of COPD and to confirm the hypothesis that elastin exposure might serve as an antigen to initiate the stimulation of CD4 ⁺ Th1-CXCR3 immune inflammation pathway. Patients of COPD in different stages and normal individuals were enrolled. Ten millilitres of peripheral blood was drawn from patients. The concentration of CXCR3, IFN-γ, IL-2, IL-4 and IL-13 in plasma was detected by ELISA. The Naïve CD4⁺T cells were isolated from the peripheral blood mononuclear cells, which were stimulated by elastin and collagen before determining the level of IFN-γ secretion by ELISPOT. Compared with control group, the concentration of CXCR3 in the acute exacerbation COPD (AECOPD) group was higher (P <.05). The concentration of IFN-γ and IL-2 in AECOPD group was lower than that in remission (P <.05). The concentration of IFN-γ in the AECOPD and remission was higher than that in controls (P <.05), while IL-2 was opposite (P <.01). The concentration of IL-4 and IL-13 in AECOPD group was higher than that in the controls (P <.05). The CD4⁺Th1 cells stimulated by the elastin as antigen secreted more IFN-γ than that by collagen (P <.01). CXCR3 was highly expressed in patients with COPD. There were different Th1/Th2 cytokines in different stages of COPD. The CD4+Th1-specific conversion and activation may be an initiator of COPD immune inflammatory response.