TY - JOUR
T1 - The investigation of solid and hollow electrode APPJs in discharge characteristics and anticancer effects
AU - Qi, Miao
AU - Peng, Sansan
AU - Fan, Runze
AU - Zhang, Xinying
AU - Pang, Bolun
AU - Liu, Zhijie
AU - Zhang, Hao
AU - Liu, Dingxin
AU - Xu, Dehui
AU - Kong, Michael G.
N1 - Publisher Copyright:
© 2022 Wiley-VCH GmbH.
PY - 2023/1
Y1 - 2023/1
N2 - Cold atmospheric pressure plasmas (CAPs) have a promising application in cancer treatment, the needle-ring electrodes plasma jet is one of the common CAPs sources. Here, we compared the discharge characteristics and anticancer effects in two plasma jets: solid electrode and hollow electrode with six working gases (He, He + 0.5%O2, He + 0.5%N2, Ar, Ar + 0.5%O2, and Ar + 0.5%N2). The results show the solid plasma jet is slightly stronger according to discharge image, optical emission spectroscopy, and concentration of H2O2 and NO2−. We used the leukemia cell THP-1 as the model to detect the anticancer effect, cell viability assay demonstrated a solid electrode plasma jet with He + 0.5%N2 has less cell survival. Moreover, apoptosis increased significantly as the plasma treatment time and Cleaved-PARP1 upregulated, SOD1 and p21 downregulated in a time-dependent manner. Our work suggested that a solid electrode plasma jet with He + 0.5%N2 may be a novel therapeutic method for acute myeloid leukemia.
AB - Cold atmospheric pressure plasmas (CAPs) have a promising application in cancer treatment, the needle-ring electrodes plasma jet is one of the common CAPs sources. Here, we compared the discharge characteristics and anticancer effects in two plasma jets: solid electrode and hollow electrode with six working gases (He, He + 0.5%O2, He + 0.5%N2, Ar, Ar + 0.5%O2, and Ar + 0.5%N2). The results show the solid plasma jet is slightly stronger according to discharge image, optical emission spectroscopy, and concentration of H2O2 and NO2−. We used the leukemia cell THP-1 as the model to detect the anticancer effect, cell viability assay demonstrated a solid electrode plasma jet with He + 0.5%N2 has less cell survival. Moreover, apoptosis increased significantly as the plasma treatment time and Cleaved-PARP1 upregulated, SOD1 and p21 downregulated in a time-dependent manner. Our work suggested that a solid electrode plasma jet with He + 0.5%N2 may be a novel therapeutic method for acute myeloid leukemia.
KW - APPJ
KW - biology anticancer mechanism
KW - hollow electrode
KW - solid electrode
UR - https://www.scopus.com/pages/publications/85137774585
U2 - 10.1002/ppap.202200095
DO - 10.1002/ppap.202200095
M3 - 文章
AN - SCOPUS:85137774585
SN - 1612-8850
VL - 20
JO - Plasma Processes and Polymers
JF - Plasma Processes and Polymers
IS - 1
M1 - 2200095
ER -
