Abstract
AIM: To investigate the inhibitory effects of dumb-bell decoy oligodeoxynucleotides(ODNs) targeting NF-kappaB on the transcriptional activity of NF-kappaB binding to its cis-elements, growth of multiple myeloma cells 8266 and the expression of IL-6 in 8266 cells. METHODS: The inhibitory effects of dumbbell decoy ODNs targeting NF-kappaB on the transcriptional activity of NF-kappaB was detected by electrophoretic mobility shift assay(EMSA). 8266 cells were randomly divided into 8266 cells of passage (control), dumb-bell decoy ODNs treatment, and cationic liposome treatment groups. Dumb-bell decoy ODNs of 2, 4 and 8 mg/L were cultured respectively into 8266 cells via cationic liposome vector. Then, the transfected 8266 cells and their supernatant were collected at 8, 12 and 18 h. IL-6 expression was measured by ELISA. Effect of decoy ODNs on the growth of 8266 cells stimulated with IL-6 was measured by MTT Colorimetry assay. RESULTS: Dumbbell decoy ODNs effectively inhibited the binding of NF-kappaB to its cis-elements in vitro. Different doses of decoy ODNs (2, 4 and 8 mg/L) had different inhibitory effects on IL-6 expression in 8266 cells. After decoy ODNs had been transfected into 8266 cells stimulated by IL-6 via cationic liposome vector, both the growth of 8266 cells and activity of IL-6 were inhibited. CONCLUSION: Dumb-bell decoy ODNs can effectively inhibit the growth of 8266 cells and decrease the expression of IL-6 in myeloma cells through the inhibiting NF-kappaB activity.
| Original language | English |
|---|---|
| Pages (from-to) | 47-50 |
| Number of pages | 4 |
| Journal | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology |
| Volume | 22 |
| Issue number | 1 |
| State | Published - Jan 2006 |