The impact of splenectomy combined with cyclosporine A on the survival of the concordant cardiac xenotransplantation

Objective: To investigate the impact of splenectomy combined with cyclosporine A on the survival time of the concordant cardiac xenotransplantation. Methods: Concordant cardiac xenotransplantation models were constructed in Hamster and SD rats. The rats were divided into different groups according to different treatment. The survival time of the xenografts was observed. The complement activity was detected by CH50 method. The xenoreactive IgG and IgM were monitored by flow cytometry. The pathology and deposition of xenoreactive IgG and IgM on the xenograft were observed. Results: The survival time of the xenografts was prolonged significantly by splenctomy combined with cyclosporine A, and the xenoreactive antibodies decreased remarkably. Conclusion: The xenoreactive antibody could be decreased through splenectomy combined with cyclosporine A early after xenotransplantation, and the survival time of the xenografts was prolonged.