Abstract
Objective: To explore the mechanism of fluoride by studying the effect of fluoride on the expression of bone morphogenetic protein(BMP) in the human early-stage tooth germ. Methods: Decidous tooth germ from human embryo of 4 months was cultured with RPMI1640 medium for 8 days. The expression of BMP was observed in every 2 days when presecretory stage tooth germ was treated with 25 mg·L-1 F- and 50 mg·L-1 F-. Image analysis apparatus was used to analyse the grey value of the cells. Results: The expression of BMP was seen primarily in enamel organ cells. BMP was expressed in the ameloblast, the intermedium stratum cell and the stellate reticulum cell but not in dental papillae cells or odontoblast. The expression of BMP significantly increased after the 6th day when 25 mg·L-1 F- was added. When 50 mg·L-1 F- was added the BMP expression significantly increased from the 2th day to the 6th day. On the 8th day the expression of BMP significantly decreased. Conclusion: Fluoride may play a certain role in the development of tooth germ through promoting one or more isoforms of BMP.
| Original language | English |
|---|---|
| Pages (from-to) | 124-126+143 |
| Journal | Journal of Xi'an Jiaotong University (Medical Sciences) |
| Volume | 24 |
| Issue number | 2 |
| State | Published - Apr 2003 |
| Externally published | Yes |
Keywords
- Ameloblast
- Bone morphogenetic protein (BMP)
- Fluoride
- Human tooth germ