Abstract
Objective: To construct HPV-6/11 L1/E6 and HPV-6/11 L1/E7 prophylactic and therapeutic DNA vaccine plasmid. Methods: HPV-6/11 L1, E6 and E7 sequences were amplified by PCR, and PCR products were inserted into the pGEM T-Easy vector and identified by sequencing. Then they were cloned into eukaryotic expression vector pVAX, cleaved by restriction enzymes and ligased to construct HPV-6/11 L1-E6/E7-pVAX plasmid. The constructed plasmid was transfected into COS-7 cell to detect the protein expression by immunohistochemistry. Results: Sequences in the constructed plasmids were correct; in cytoplasma, sometimes in nucleus, yellow positive products were found with immunohistochemistry. Conclusion: The constructed fusion protein expression plasmid HPV-6/11 L1-E6/E7-pVAX can express L1-E6/L1-E7 protein in vitro, which has paved a way for animal experiment and clinical study in the future.
| Original language | English |
|---|---|
| Pages (from-to) | 413-415+442 |
| Journal | Journal of Xi'an Jiaotong University (Medical Sciences) |
| Volume | 24 |
| Issue number | 5 |
| State | Published - Oct 2003 |
Keywords
- Cervical cancer
- DNA vaccine
- HPV-6/11
