The analysis of vital reaction following skin injury close to death in mouse by FTIR

Fourier transform infrared spectroscopy (FTIR) was applied to study the difference of vital reaction following skin injury close to death in mouse and provided the evidence for forensic practice. The results demonstrated that vital 5 min, postmortem and normal groups showed significant differences in the wave form and intensity: (1) The intensity at 3007 and 1745 cm^-1 related to lipid for the vital injury was higher than that for the postmortem; (2) The intensity at 1160 cm^-1 related to carbohydrate for the vital injure increased more compared to the postmortem; (3) The intensity of the band at 1640 cm^-1 related to amide I band for the injury groups was higher than the normal, but it was the lowest for the vital injury. These results show that it will be possible for FTIR to become an effective method to determine the vital reaction of injury close to death in forensic practice.