Synthesis, characterization, and DNA-binding of chiral complexes Δ- and Λ-[Ru(bpy)2(pyip)]2+

Shuo Shi; Tian Ming Yao; Xiao Ting Geng; Ling Feng Jiang; Jie Liu; Qing Yuan Yang; Liang Nian Ji

doi:10.1002/chir.20573

Synthesis, characterization, and DNA-binding of chiral complexes Δ- and Λ-[Ru(bpy)₂(pyip)]²⁺

Shuo Shi
, Tian Ming Yao
, Xiao Ting Geng
, Ling Feng Jiang
, Jie Liu
, Qing Yuan Yang
, Liang Nian Ji

Tongji University
Sun Yat-Sen University

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

New chiral Ru(II) complexes Δ and Λ-[Ru(bpy)₂(pyip)](PF₆)₂ [(bpy = 2,2′-bipyridine; pyip = (2-(1-pyrenyl)-1H-imidazo[4,5-f] [1,10]phenanthroline] were synthesized and characterized by elemental analysis, ¹H NMR, ESI-MS, IR, and CD spectra. Their DNA-binding properties were studied by means of UV-vis, emission spectra, CD spectra and viscosity measurements. A subtle but detectable difference was observed in the interaction of both enantiomer with CT-DNA. Spectroscopy experiments indicated that each of these complexes could interact with the DNA. The DNA-binding of the Δ-enantiomer was stronger than that of Λ-enantiomer. DNA-viscosity experiments provided evidence that both Δ- and Λ-[Ru(bpy)₂(pyip)](PF₆)₂ bound to DNA by intercalation. At the same time, the DNA-photocleavage properties of the complexes were investigated too. Under irradiation with UV light, Ru(II) complexes showed different efficiency of cleaving DNA.

Original language	English
Pages (from-to)	276-283
Number of pages	8
Journal	Chirality
Volume	21
Issue number	2
DOIs	https://doi.org/10.1002/chir.20573
State	Published - 2009
Externally published	Yes

Keywords

DNA-binding
Enantiomer
Photo-cleavage
Ru(II) complex
Stereoselectivity

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10.1002/chir.20573

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@article{1538b45d97884eb58dfff56169a12db6,

title = "Synthesis, characterization, and DNA-binding of chiral complexes Δ- and Λ-[Ru(bpy)2(pyip)]2+",

abstract = "New chiral Ru(II) complexes Δ and Λ-[Ru(bpy)2(pyip)](PF6)2 [(bpy = 2,2′-bipyridine; pyip = (2-(1-pyrenyl)-1H-imidazo[4,5-f] [1,10]phenanthroline] were synthesized and characterized by elemental analysis, 1H NMR, ESI-MS, IR, and CD spectra. Their DNA-binding properties were studied by means of UV-vis, emission spectra, CD spectra and viscosity measurements. A subtle but detectable difference was observed in the interaction of both enantiomer with CT-DNA. Spectroscopy experiments indicated that each of these complexes could interact with the DNA. The DNA-binding of the Δ-enantiomer was stronger than that of Λ-enantiomer. DNA-viscosity experiments provided evidence that both Δ- and Λ-[Ru(bpy)2(pyip)](PF6)2 bound to DNA by intercalation. At the same time, the DNA-photocleavage properties of the complexes were investigated too. Under irradiation with UV light, Ru(II) complexes showed different efficiency of cleaving DNA.",

keywords = "DNA-binding, Enantiomer, Photo-cleavage, Ru(II) complex, Stereoselectivity",

author = "Shuo Shi and Yao, \{Tian Ming\} and Geng, \{Xiao Ting\} and Jiang, \{Ling Feng\} and Jie Liu and Yang, \{Qing Yuan\} and Ji, \{Liang Nian\}",

year = "2009",

doi = "10.1002/chir.20573",

language = "英语",

volume = "21",

pages = "276--283",

journal = "Chirality",

issn = "0899-0042",

publisher = "Wiley-Liss Inc.",

number = "2",

}

TY - JOUR

T1 - Synthesis, characterization, and DNA-binding of chiral complexes Δ- and Λ-[Ru(bpy)2(pyip)]2+

AU - Shi, Shuo

AU - Yao, Tian Ming

AU - Geng, Xiao Ting

AU - Jiang, Ling Feng

AU - Liu, Jie

AU - Yang, Qing Yuan

AU - Ji, Liang Nian

PY - 2009

Y1 - 2009

N2 - New chiral Ru(II) complexes Δ and Λ-[Ru(bpy)2(pyip)](PF6)2 [(bpy = 2,2′-bipyridine; pyip = (2-(1-pyrenyl)-1H-imidazo[4,5-f] [1,10]phenanthroline] were synthesized and characterized by elemental analysis, 1H NMR, ESI-MS, IR, and CD spectra. Their DNA-binding properties were studied by means of UV-vis, emission spectra, CD spectra and viscosity measurements. A subtle but detectable difference was observed in the interaction of both enantiomer with CT-DNA. Spectroscopy experiments indicated that each of these complexes could interact with the DNA. The DNA-binding of the Δ-enantiomer was stronger than that of Λ-enantiomer. DNA-viscosity experiments provided evidence that both Δ- and Λ-[Ru(bpy)2(pyip)](PF6)2 bound to DNA by intercalation. At the same time, the DNA-photocleavage properties of the complexes were investigated too. Under irradiation with UV light, Ru(II) complexes showed different efficiency of cleaving DNA.

AB - New chiral Ru(II) complexes Δ and Λ-[Ru(bpy)2(pyip)](PF6)2 [(bpy = 2,2′-bipyridine; pyip = (2-(1-pyrenyl)-1H-imidazo[4,5-f] [1,10]phenanthroline] were synthesized and characterized by elemental analysis, 1H NMR, ESI-MS, IR, and CD spectra. Their DNA-binding properties were studied by means of UV-vis, emission spectra, CD spectra and viscosity measurements. A subtle but detectable difference was observed in the interaction of both enantiomer with CT-DNA. Spectroscopy experiments indicated that each of these complexes could interact with the DNA. The DNA-binding of the Δ-enantiomer was stronger than that of Λ-enantiomer. DNA-viscosity experiments provided evidence that both Δ- and Λ-[Ru(bpy)2(pyip)](PF6)2 bound to DNA by intercalation. At the same time, the DNA-photocleavage properties of the complexes were investigated too. Under irradiation with UV light, Ru(II) complexes showed different efficiency of cleaving DNA.

KW - DNA-binding

KW - Enantiomer

KW - Photo-cleavage

KW - Ru(II) complex

KW - Stereoselectivity

UR - https://www.scopus.com/pages/publications/60549106452

U2 - 10.1002/chir.20573

DO - 10.1002/chir.20573

M3 - 文章

C2 - 18537162

AN - SCOPUS:60549106452

SN - 0899-0042

VL - 21

SP - 276

EP - 283

JO - Chirality

JF - Chirality

IS - 2

ER -

Synthesis, characterization, and DNA-binding of chiral complexes Δ- and Λ-[Ru(bpy)₂(pyip)]²⁺

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