Study on fabrication of controllable microchannel structure scaffolds and rotating dynamic culture

It is the critical technology for bone tissue engineering to fabricate scaffolds, especially to fabricate the internal microchannel of scaffolds. Utilizing stereolithography rapid prototyping indirect fabrication approach, suitable size microchannel structure scaffolds with orientation, branching, and interconnectivity under control was manufactured. Observation under light microscope showed that the arrange of microchannel size was 200-600 microm, the orientation and branching were accorded with designed structure, and all the microchannels were completely interconnected. Scaffolds seeded with osteoblast cells were put into 6-well plate and rotating 3D dynamic culture system which was developed in our lab. The morphology of osteoblasts and the development on the surface and internal microchannel of scaffolds were observed under scanning electron microscope (SEM). The results indicated that the dynamic culture permited an even distribution of cells across the surface of scaffolds, besides, a few cells grew into the lumen of microchannels.