Studies on gallbladder tissues, tissue plasma membranes and cell lines by FTIR

12 Cancer tissue samples, 14 inflammatory samples and 10 normal samples of gallbladder and their corresponding tissue membranes and gallbladder cancer cell lines were observed via FTIR. Compared to normal gallbladder tissue, the intensities of bands, related to lipids, at 2955, 2920, 2870, 2850 and 1740 cm^-1, decreased for gallbladder cancer tissue, the intensity of bands, related to protein, at 1550 cm-1 decreased for gallbladder cancer tissue; the intensities of lipids bands and protein bands increased for gallbladder cancer cell line obviously; the intensities of lipids related bands also increased for gallbladder cancer tissue membrane; the intensity of band related to nucleic acid for gallbladder cancer tissue is similar to that for gallbladder cancer cell line and the band disappeared for gallbladder cancer tissue plasma membrane; the ratio of I₁₄₆₀/I₁₄₀₀ changed uniformity for tissue, cell line and tissue plasma membrane, which is smaller for cancer ones and bigger for normal ones. These results indicate that compared with those of gallbladder cancer tissue, the FTIR spectra changed a lot for gallbladder cancer cell lines and gallbladder cancer tissue plasma membrane. Protein and lipids relatively decreased in gallbladder cancer tissues but increased in gallbladder cancer cell line and gallbladder cancer tissue plasma membrane. The paper expounds FTIR variation for carcinogenesis from tissue, cell line and tissue plasma membrane. The ratio of I₁₄₆₀/I₁₄₀₀ changed uniformity for tissue, cell line and tissue plasma membrane, more studies may get a fine clinical diagnosis index.