Stimulation of dorsal root ganglion neurons activity by pancreatic cancer cell lines

Jun Hui Li; Qing Yong Ma; Su Gang Shen; Heng Tong Hu

doi:10.1016/j.cellbi.2008.08.022

Stimulation of dorsal root ganglion neurons activity by pancreatic cancer cell lines

Jun Hui Li
, Qing Yong Ma
, Su Gang Shen
, Heng Tong Hu

Health Science Center

Medical College

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Stimulation of mice dorsal root ganglion neurons (DRGNs) activity by human pancreatic cancer (PanCa) cell line Mia PaCa-2 and its potential molecule mechanism has been investaged. DRGNs were cultured alone or along with the MIA PaCa-2. The effects of MIA PaCa-2 to DRGNs were determined by neurofilament (NF) immunocytochemical and Nissl staining. ELISA was used to detect the concentration of insulin-like growth factor-1 (IGF-1) in the culture supernatant. Cyton size, neurite outgrowth and neuronal activity in the experimental group were greater than in the control groups. However, the concentration of IGF-1 in the supernatants was not significantly different from those in the blank and non-cultured medium groups. In the presence of MIA PaCa-2 cell line, cyton size, neurite outgrowth and neuronal activity were enhanced, which may provide more routes for the invasion of cancer cells along nerves.

Original language	English
Pages (from-to)	1530-1535
Number of pages	6
Journal	Cell Biology International
Volume	32
Issue number	12
DOIs	https://doi.org/10.1016/j.cellbi.2008.08.022
State	Published - Dec 2008

Keywords

Cell coculture
DRGNs activity
Neurites outgrowth
Neuron cyton size
Pancreatic cancer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.cellbi.2008.08.022

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@article{a38cb2ee2c2e4880b84a1209996493f6,

title = "Stimulation of dorsal root ganglion neurons activity by pancreatic cancer cell lines",

abstract = "Stimulation of mice dorsal root ganglion neurons (DRGNs) activity by human pancreatic cancer (PanCa) cell line Mia PaCa-2 and its potential molecule mechanism has been investaged. DRGNs were cultured alone or along with the MIA PaCa-2. The effects of MIA PaCa-2 to DRGNs were determined by neurofilament (NF) immunocytochemical and Nissl staining. ELISA was used to detect the concentration of insulin-like growth factor-1 (IGF-1) in the culture supernatant. Cyton size, neurite outgrowth and neuronal activity in the experimental group were greater than in the control groups. However, the concentration of IGF-1 in the supernatants was not significantly different from those in the blank and non-cultured medium groups. In the presence of MIA PaCa-2 cell line, cyton size, neurite outgrowth and neuronal activity were enhanced, which may provide more routes for the invasion of cancer cells along nerves.",

keywords = "Cell coculture, DRGNs activity, Neurites outgrowth, Neuron cyton size, Pancreatic cancer",

author = "Li, \{Jun Hui\} and Ma, \{Qing Yong\} and Shen, \{Su Gang\} and Hu, \{Heng Tong\}",

year = "2008",

month = dec,

doi = "10.1016/j.cellbi.2008.08.022",

language = "英语",

volume = "32",

pages = "1530--1535",

journal = "Cell Biology International",

issn = "1065-6995",

publisher = "Wiley-Blackwell",

number = "12",

}

TY - JOUR

T1 - Stimulation of dorsal root ganglion neurons activity by pancreatic cancer cell lines

AU - Li, Jun Hui

AU - Ma, Qing Yong

AU - Shen, Su Gang

AU - Hu, Heng Tong

PY - 2008/12

Y1 - 2008/12

N2 - Stimulation of mice dorsal root ganglion neurons (DRGNs) activity by human pancreatic cancer (PanCa) cell line Mia PaCa-2 and its potential molecule mechanism has been investaged. DRGNs were cultured alone or along with the MIA PaCa-2. The effects of MIA PaCa-2 to DRGNs were determined by neurofilament (NF) immunocytochemical and Nissl staining. ELISA was used to detect the concentration of insulin-like growth factor-1 (IGF-1) in the culture supernatant. Cyton size, neurite outgrowth and neuronal activity in the experimental group were greater than in the control groups. However, the concentration of IGF-1 in the supernatants was not significantly different from those in the blank and non-cultured medium groups. In the presence of MIA PaCa-2 cell line, cyton size, neurite outgrowth and neuronal activity were enhanced, which may provide more routes for the invasion of cancer cells along nerves.

AB - Stimulation of mice dorsal root ganglion neurons (DRGNs) activity by human pancreatic cancer (PanCa) cell line Mia PaCa-2 and its potential molecule mechanism has been investaged. DRGNs were cultured alone or along with the MIA PaCa-2. The effects of MIA PaCa-2 to DRGNs were determined by neurofilament (NF) immunocytochemical and Nissl staining. ELISA was used to detect the concentration of insulin-like growth factor-1 (IGF-1) in the culture supernatant. Cyton size, neurite outgrowth and neuronal activity in the experimental group were greater than in the control groups. However, the concentration of IGF-1 in the supernatants was not significantly different from those in the blank and non-cultured medium groups. In the presence of MIA PaCa-2 cell line, cyton size, neurite outgrowth and neuronal activity were enhanced, which may provide more routes for the invasion of cancer cells along nerves.

KW - Cell coculture

KW - DRGNs activity

KW - Neurites outgrowth

KW - Neuron cyton size

KW - Pancreatic cancer

UR - https://www.scopus.com/pages/publications/55949120103

U2 - 10.1016/j.cellbi.2008.08.022

DO - 10.1016/j.cellbi.2008.08.022

M3 - 文章

C2 - 18801449

AN - SCOPUS:55949120103

SN - 1065-6995

VL - 32

SP - 1530

EP - 1535

JO - Cell Biology International

JF - Cell Biology International

IS - 12

ER -

Stimulation of dorsal root ganglion neurons activity by pancreatic cancer cell lines

Abstract

Keywords

UN SDGs

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