Sensitive and specific detection of peroxynitrite and: In vivo imaging of inflammation by a "simple" AIE bioprobe

Huilin Xie; Jingtian Zhang; Chao Chen; Feiyi Sun; Haixiang Liu; Xinyuan He; Kristy W.K. Lam; Zhao Li; Jacky W.Y. Lam; Guo Qiang Zhang; Dan Ding; Ryan T.K. Kwok; Ben Zhong Tang

doi:10.1039/d0qm01004a

Sensitive and specific detection of peroxynitrite and: In vivo imaging of inflammation by a "simple" AIE bioprobe

Huilin Xie
, Jingtian Zhang
, Chao Chen
, Feiyi Sun
, Haixiang Liu
, Xinyuan He
, Kristy W.K. Lam
, Zhao Li
, Jacky W.Y. Lam
, Guo Qiang Zhang
, Dan Ding
, Ryan T.K. Kwok
, Ben Zhong Tang

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

A luminogenic bioprobe TPE-DMAB for simple and specific detection of peroxynitrite (ONOO-) has been developed. TPE-DMAB exhibits aggregation-induced emission (AIE) characteristic and shows fluorescence enhancement (up to 100-fold) upon cleavage of phenylboronic moieties by ONOO-. TPE-DMAB can be facilely synthesized and displays excellent specificity and sensitivity towards ONOO- (detection limit of 54 nM). Based on these novel properties of TPE-DMAB, we further produced its encapsulated nanoparticles and successfully used them to detect endogenous ONOO- in macrophage cells and visualize the inflammation sites of mice, verifying the great potential of TPE-DMAB for biological applications.

Original language	English
Pages (from-to)	1830-1835
Number of pages	6
Journal	Materials Chemistry Frontiers
Volume	5
Issue number	4
DOIs	https://doi.org/10.1039/d0qm01004a
State	Published - 21 Feb 2021
Externally published	Yes

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10.1039/d0qm01004a

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title = "Sensitive and specific detection of peroxynitrite and: In vivo imaging of inflammation by a {"}simple{"} AIE bioprobe",

abstract = "A luminogenic bioprobe TPE-DMAB for simple and specific detection of peroxynitrite (ONOO-) has been developed. TPE-DMAB exhibits aggregation-induced emission (AIE) characteristic and shows fluorescence enhancement (up to 100-fold) upon cleavage of phenylboronic moieties by ONOO-. TPE-DMAB can be facilely synthesized and displays excellent specificity and sensitivity towards ONOO- (detection limit of 54 nM). Based on these novel properties of TPE-DMAB, we further produced its encapsulated nanoparticles and successfully used them to detect endogenous ONOO- in macrophage cells and visualize the inflammation sites of mice, verifying the great potential of TPE-DMAB for biological applications.",

author = "Huilin Xie and Jingtian Zhang and Chao Chen and Feiyi Sun and Haixiang Liu and Xinyuan He and Lam, \{Kristy W.K.\} and Zhao Li and Lam, \{Jacky W.Y.\} and Zhang, \{Guo Qiang\} and Dan Ding and Kwok, \{Ryan T.K.\} and Tang, \{Ben Zhong\}",

note = "Publisher Copyright: {\textcopyright} the Partner Organisations.",

year = "2021",

month = feb,

day = "21",

doi = "10.1039/d0qm01004a",

language = "英语",

volume = "5",

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journal = "Materials Chemistry Frontiers",

issn = "2052-1537",

publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Sensitive and specific detection of peroxynitrite and

T2 - In vivo imaging of inflammation by a "simple" AIE bioprobe

AU - Xie, Huilin

AU - Zhang, Jingtian

AU - Chen, Chao

AU - Sun, Feiyi

AU - Liu, Haixiang

AU - He, Xinyuan

AU - Lam, Kristy W.K.

AU - Li, Zhao

AU - Lam, Jacky W.Y.

AU - Zhang, Guo Qiang

AU - Ding, Dan

AU - Kwok, Ryan T.K.

AU - Tang, Ben Zhong

PY - 2021/2/21

Y1 - 2021/2/21

N2 - A luminogenic bioprobe TPE-DMAB for simple and specific detection of peroxynitrite (ONOO-) has been developed. TPE-DMAB exhibits aggregation-induced emission (AIE) characteristic and shows fluorescence enhancement (up to 100-fold) upon cleavage of phenylboronic moieties by ONOO-. TPE-DMAB can be facilely synthesized and displays excellent specificity and sensitivity towards ONOO- (detection limit of 54 nM). Based on these novel properties of TPE-DMAB, we further produced its encapsulated nanoparticles and successfully used them to detect endogenous ONOO- in macrophage cells and visualize the inflammation sites of mice, verifying the great potential of TPE-DMAB for biological applications.

AB - A luminogenic bioprobe TPE-DMAB for simple and specific detection of peroxynitrite (ONOO-) has been developed. TPE-DMAB exhibits aggregation-induced emission (AIE) characteristic and shows fluorescence enhancement (up to 100-fold) upon cleavage of phenylboronic moieties by ONOO-. TPE-DMAB can be facilely synthesized and displays excellent specificity and sensitivity towards ONOO- (detection limit of 54 nM). Based on these novel properties of TPE-DMAB, we further produced its encapsulated nanoparticles and successfully used them to detect endogenous ONOO- in macrophage cells and visualize the inflammation sites of mice, verifying the great potential of TPE-DMAB for biological applications.

UR - https://www.scopus.com/pages/publications/85101326290

U2 - 10.1039/d0qm01004a

DO - 10.1039/d0qm01004a

M3 - 文章

AN - SCOPUS:85101326290

SN - 2052-1537

VL - 5

SP - 1830

EP - 1835

JO - Materials Chemistry Frontiers

JF - Materials Chemistry Frontiers

IS - 4

ER -

Sensitive and specific detection of peroxynitrite and: In vivo imaging of inflammation by a "simple" AIE bioprobe

Abstract

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