Repetitive application of recombinant Pla a 1 reduces the airway inflammation and hyperresponsiveness in P. Acerifolia sensitized mice

Purpose: To evaluate whether repetitive injection of rPla a 1 can decrease the airway inflammation and hyperresponsiveness in P. acerifolia sensitized mice. Materials and Methods: After sensitization, mice were injected intraperitoneally with rPla a 1 for five times. Lung tissues from the mice were collected for histopathology analysis, period acid-schiff (PAS) and in situ hybridization (ISH) to detect the expression of IL-10 and FoxP3. Airway responsiveness was assessed in the whole body plethysmograph. Bronchoalveolar lavage fluids (BALF) were harvested from the mice to analyze the composition of inflammatory cells. Blood samples were collected to measure levels of P. acerifolia specific IgE (sIgE) and IgG (sIgG). Additionally, cytokine productions (IL-10, IL-13 and IL-17) from the spleen tissue homogenate were measured. Results: Intraperitoneal administration of rPla a 1 attenuated the airway inflammation and mucus production caused by P. acerifolia sensitization, reduced the airway responsiveness, and promoted the expression of IL-10 and FoxP3 in the lungs. In addition, intraperitoneal administration of rPla a 1 notably induced the production of sIgG in the serum and decreased IL-13 and IL-17 in the spleen tissue homogenate. Conclusion: Repetitive application of rPla a 1 reduces the airway inflammation and hyperresponsiveness in P. acerifolia sensitized mice, which may serve as an impetus for further research on the use of recombinant protein in the management of human allergic asthma.