Relationship of intrauterine HCMV infection with UL54 and UL97 gene mutation in Qinba mountainous area

Objective: To analyze the relationship of intrauterine human cytomegalovirus (HCMV) infection with UL54 and UL97 gene mutation in pregnant women in Qinba mountainous area. Methods: HCMV-DNA was detected with PCR in the sera of 228 pregnant women and umbilical cord blood of their infants. For subjects with positive DNA, restriction fragment length polymorphism (RFLP) was applied to detect whether 501, 594 and 595 codes in UL54 and UL97 gene mutated. Results: Circled digit one The HCMV-DNA positive rate in the sera of pregnant women was 8.33%, and 3.51% in that of neonates. In the 19 pregnant women with positive HCMV DNA, 7 of them were confirmed to be infected with HCMV in umbilical cord blood of neonates. The rate of intrauterine HCMV infection was 36.84%. Circled digit two 501 and 594 and 595 codes in UL54 and UL97 gene were not found to mutate in the sera of pregnant women and umbilical cord blood of neonates with positive HCMV DNA. Conclusion: The rate of intrauterine HCMV infection was high, but the infection was not related to 501, 594 and 595 code mutation of UL54 and UL97 gene in Qinba mountainous area, but the possibility of change by mutation in other gene codes or by other factors cannot be excluded.