Abstract
Objective: To investigate microbiome dysbiosis of intestinal flora in experimental uremic rats and its correlation with microinflammation. Methods: Healthy male SD rats were randomly divided into uremic group and sham group. Uremic model was established in uremic group by 5/6 nephrectomy, while sham group underwent nephrocapsulotomy. All rats were sacrificed at the end of week 10. Serum creatinine, urea nitrogen, IL-6 and CRP were examined. Histopathological changes of rat kidney were observed by HE and Masson staining. The numbers of Bifidobacterium, Lactobacillus and E.coli in the intestinal tract (jejunum, ileum and colon) were quantitatively determined by real-time fluorescent quantitative PCR. Results: Serum IL-6 and CRP were significantly increased in uremic group (P<0.05). Compared with sham group, the number of E.coli in the jejunum increased significantly (P<0.05) while that of Bifidobacterium and Lactobaeillus in the ileum decreased (P<0.05). The number of Bifidobacterium and Lactobaeillus decreased in the colon whereas that of E.coli increased (P<0.05). Correlation analysis showed that the number of E.coli in the colon had a positive correlation with IL-6 in uremic group. Conclusion: Microbiome dysbiosis of intestinal flora occurs in uremic rats and correlates with microinflammation. The former one may be one of the causes of the latter.
| Original language | English |
|---|---|
| Pages (from-to) | 341-345 |
| Number of pages | 5 |
| Journal | Journal of Xi'an Jiaotong University (Medical Sciences) |
| Volume | 34 |
| Issue number | 3 |
| State | Published - May 2013 |
Keywords
- Bun
- CRP
- Gut
- IL-6
- Microbiome dysbiosis
- Microinflammation
- Scr
- Uremia