[Regulation of chondrosarcoma cell growth using synthesized hydrogels with different electric charges].

  • Yulong Han
  • , Zhenqi Liu
  • , Baoyong Sha
  • , Lin Wang
  • , Lihong Zhou
  • , Yongmei Chen
  • , Zhenfeng Duan
  • , Tianjian Lu
  • , Feng Xu

Research output: Contribution to journalArticlepeer-review

Abstract

To develop standard in vitro chondrosarcoma models, we synthesized three hydrogels (i. e., PDMAAm, PNaAMPS and PMETAC) and investigated the influence of Young's modulus, swelling ratio and electric charges on the behavior of chondrosarcoma cells seeded on the hydrogels, including morphology, adhesion and aggregation. Results showed that the morphology of chondrosarcoma cells at 6h was dependent on the charges of hydrogels; cells present spindle-shaped and round-shaped morphology on negative charged and neutral hydrogel, respectively, while no cells spreaded on positive charged hydrogel. Chondrosarcoma cells formed aggregates on neutral PDMAAm after further culture. The hydrogels can be synthesized easily and has the characteristics of ease at use with defined components, which holds great potential for developing standard chondrosarcoma models in vitro.

Original languageEnglish
Pages (from-to)782-788
Number of pages7
JournalSheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Volume30
Issue number4
StatePublished - Aug 2013

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