Promoting the formation and stabilization of G-quadruplex by dinuclear RuII complex Ru2(obip)L4

Shuo Shi; Jie Liu; Tianming Yao; Xiaoting Geng; Lingfeng Jiang; Qingyuan Yang; Lin Cheng; Liangnian Ji

doi:10.1021/ic7021209

Promoting the formation and stabilization of G-quadruplex by dinuclear Ru^II complex Ru₂(obip)L₄

Shuo Shi
, Jie Liu
, Tianming Yao
, Xiaoting Geng
, Lingfeng Jiang
, Qingyuan Yang
, Lin Cheng
, Liangnian Ji

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

The remarkable ability of a new dinuclear Ru^II complex Ru ₂(obip)L₄ [obip = 2-(2-pyridyl)imidazo[4,5-f][1,10]- phenanthroline; L = 2,2′-bipyridine] to promote the formation and stabilization of the human telomeric repeat AG3(T2AG3)3 quadruplex was reported. The experimental results indicated that Ru₂(obip)L₄ could induce the formation of an antiparallel G-quadruplex structure in the absence of metal cations. It could induce positive T_m shifts of +9.4 and +5.8°C in Na⁺ and K⁺ buffers, respectively, in which an increase in the melting temperature of the quadruplex indicated a stabilizing effect. Binding stoichiometry with the quadruplex was investigated through a luminescence-based Job plot. The major inflection point for Ru ₂(obip)L₄ at x = 0.48 was observed. The data were consistent with a 1:1 [quadruplex]/[complex] binding mode, which was suggestive of a specific Ru₂(obip)L₄-quadruplex interaction with a single guanine tetrad.

Original language	English
Pages (from-to)	2910-2912
Number of pages	3
Journal	Inorganic Chemistry
Volume	47
Issue number	8
DOIs	https://doi.org/10.1021/ic7021209
State	Published - 21 Apr 2008
Externally published	Yes

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title = "Promoting the formation and stabilization of G-quadruplex by dinuclear RuII complex Ru2(obip)L4",

abstract = "The remarkable ability of a new dinuclear RuII complex Ru 2(obip)L4 [obip = 2-(2-pyridyl)imidazo[4,5-f][1,10]- phenanthroline; L = 2,2′-bipyridine] to promote the formation and stabilization of the human telomeric repeat AG3(T2AG3)3 quadruplex was reported. The experimental results indicated that Ru2(obip)L4 could induce the formation of an antiparallel G-quadruplex structure in the absence of metal cations. It could induce positive Tm shifts of +9.4 and +5.8°C in Na+ and K+ buffers, respectively, in which an increase in the melting temperature of the quadruplex indicated a stabilizing effect. Binding stoichiometry with the quadruplex was investigated through a luminescence-based Job plot. The major inflection point for Ru 2(obip)L4 at x = 0.48 was observed. The data were consistent with a 1:1 [quadruplex]/[complex] binding mode, which was suggestive of a specific Ru2(obip)L4-quadruplex interaction with a single guanine tetrad.",

author = "Shuo Shi and Jie Liu and Tianming Yao and Xiaoting Geng and Lingfeng Jiang and Qingyuan Yang and Lin Cheng and Liangnian Ji",

year = "2008",

month = apr,

day = "21",

doi = "10.1021/ic7021209",

language = "英语",

volume = "47",

pages = "2910--2912",

journal = "Inorganic Chemistry",

issn = "0020-1669",

publisher = "American Chemical Society",

number = "8",

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TY - JOUR

T1 - Promoting the formation and stabilization of G-quadruplex by dinuclear RuII complex Ru2(obip)L4

AU - Shi, Shuo

AU - Liu, Jie

AU - Yao, Tianming

AU - Geng, Xiaoting

AU - Jiang, Lingfeng

AU - Yang, Qingyuan

AU - Cheng, Lin

AU - Ji, Liangnian

PY - 2008/4/21

Y1 - 2008/4/21

N2 - The remarkable ability of a new dinuclear RuII complex Ru 2(obip)L4 [obip = 2-(2-pyridyl)imidazo[4,5-f][1,10]- phenanthroline; L = 2,2′-bipyridine] to promote the formation and stabilization of the human telomeric repeat AG3(T2AG3)3 quadruplex was reported. The experimental results indicated that Ru2(obip)L4 could induce the formation of an antiparallel G-quadruplex structure in the absence of metal cations. It could induce positive Tm shifts of +9.4 and +5.8°C in Na+ and K+ buffers, respectively, in which an increase in the melting temperature of the quadruplex indicated a stabilizing effect. Binding stoichiometry with the quadruplex was investigated through a luminescence-based Job plot. The major inflection point for Ru 2(obip)L4 at x = 0.48 was observed. The data were consistent with a 1:1 [quadruplex]/[complex] binding mode, which was suggestive of a specific Ru2(obip)L4-quadruplex interaction with a single guanine tetrad.

AB - The remarkable ability of a new dinuclear RuII complex Ru 2(obip)L4 [obip = 2-(2-pyridyl)imidazo[4,5-f][1,10]- phenanthroline; L = 2,2′-bipyridine] to promote the formation and stabilization of the human telomeric repeat AG3(T2AG3)3 quadruplex was reported. The experimental results indicated that Ru2(obip)L4 could induce the formation of an antiparallel G-quadruplex structure in the absence of metal cations. It could induce positive Tm shifts of +9.4 and +5.8°C in Na+ and K+ buffers, respectively, in which an increase in the melting temperature of the quadruplex indicated a stabilizing effect. Binding stoichiometry with the quadruplex was investigated through a luminescence-based Job plot. The major inflection point for Ru 2(obip)L4 at x = 0.48 was observed. The data were consistent with a 1:1 [quadruplex]/[complex] binding mode, which was suggestive of a specific Ru2(obip)L4-quadruplex interaction with a single guanine tetrad.

UR - https://www.scopus.com/pages/publications/43149094210

U2 - 10.1021/ic7021209

DO - 10.1021/ic7021209

M3 - 文章

C2 - 18351733

AN - SCOPUS:43149094210

SN - 0020-1669

VL - 47

SP - 2910

EP - 2912

JO - Inorganic Chemistry

JF - Inorganic Chemistry

IS - 8

ER -

Promoting the formation and stabilization of G-quadruplex by dinuclear Ru^II complex Ru₂(obip)L₄

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