TY - JOUR
T1 - Presence of HBsAg, HBcAg, and HBVDNA in ovary and ovum of the patients with chronic hepatitis B virus infection
AU - Ye, Feng
AU - Yue, Yafei
AU - Li, Shuhong
AU - Chen, Tianyan
AU - Bai, Guiqin
AU - Liu, Min
AU - Zhang, Shulin
PY - 2006/2
Y1 - 2006/2
N2 - Objective: The purpose of this study was to investigate the presence and distribution of Hepatitis B virus antigen (HBVAg) and Hepatitis B virus deoxyribonucleic acid (HBVDNA) in ovary with chronic hepatitis B virus. Study design: The immunohistochemistry method and in situ hybridization method were used to detect the hepatitis B surface antigen (HBsAg), anti-hepatitis B core antibody (HBcAg), and HBVDNA in ovary of patients with chronic hepatitis B infection. Results: HBsAg presented in the ovum-granular cell and interstitial cell of ovary. HBsAg located in the cytomembrane and cytoplasm. HBcAg presented in the ovum-granular cell-interstitial cell and endothelium cell of interstitial blood vessel of ovary, and the HBcAg may present in the ovum at different stage. HBcAg was situated at the cytomembrane, cytoplasm, and nucleus. HBVDNA was detected in the ovum at different stage as HBcAg and in granular cell, interstitial cell of ovary. In the same ovary tissue, HBVDNA was positive in some ova, but it was weakly positive or negative in other ova. HBVDNA distributed mainly in nucleus, but also can be detected in the cytoplasm. Conclusion: HBV could infect the ovum at different stage and replicate in it. This may be an important mechanism of HBV vertical transmission.
AB - Objective: The purpose of this study was to investigate the presence and distribution of Hepatitis B virus antigen (HBVAg) and Hepatitis B virus deoxyribonucleic acid (HBVDNA) in ovary with chronic hepatitis B virus. Study design: The immunohistochemistry method and in situ hybridization method were used to detect the hepatitis B surface antigen (HBsAg), anti-hepatitis B core antibody (HBcAg), and HBVDNA in ovary of patients with chronic hepatitis B infection. Results: HBsAg presented in the ovum-granular cell and interstitial cell of ovary. HBsAg located in the cytomembrane and cytoplasm. HBcAg presented in the ovum-granular cell-interstitial cell and endothelium cell of interstitial blood vessel of ovary, and the HBcAg may present in the ovum at different stage. HBcAg was situated at the cytomembrane, cytoplasm, and nucleus. HBVDNA was detected in the ovum at different stage as HBcAg and in granular cell, interstitial cell of ovary. In the same ovary tissue, HBVDNA was positive in some ova, but it was weakly positive or negative in other ova. HBVDNA distributed mainly in nucleus, but also can be detected in the cytoplasm. Conclusion: HBV could infect the ovum at different stage and replicate in it. This may be an important mechanism of HBV vertical transmission.
KW - HBV
KW - Immunohistochemistry
KW - In situ hybridization
KW - Ovary
KW - Ovum
KW - Patient with chronic hepatitis B virus infection
KW - Vertical transmission
UR - https://www.scopus.com/pages/publications/31844444876
U2 - 10.1016/j.ajog.2005.07.011
DO - 10.1016/j.ajog.2005.07.011
M3 - 文章
C2 - 16458634
AN - SCOPUS:31844444876
SN - 0002-9378
VL - 194
SP - 387
EP - 392
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
IS - 2
ER -
