Preparation and Characterization of a Novel Monoclonal Antibody Specific to Human ZNRD1 Protein

Liu Hong; Yumei Zhang; Shuang Han; Jin Wang; Yongquan Shi; Yanglin Pan; Na Liu; Xiaoyin Zhang; Daiming Fan

doi:10.1089/153685904322772042

Preparation and Characterization of a Novel Monoclonal Antibody Specific to Human ZNRD1 Protein

Liu Hong
, Yumei Zhang
, Shuang Han
, Jin Wang
, Yongquan Shi
, Yanglin Pan
, Na Liu
, Xiaoyin Zhang
, Daiming Fan

Xijing Hospital

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

We have efficiently generated the first monoclonal antibody (MAb) against the human ZNRD1 protein, a transcription-associated protein, consisting of two zinc ribbon domains. Hybridomas were screened by indirect enzyme-linked immunosorbent assay (ELISA) using either purified 6 x His-ZNRD1fusion protein or purified 6 x His-OS-9 fusion protein as a control. One MAb named H6 (IgG ₁), effective in detecting the recombinant and cellular protein, was characterized by ELISA and Western immunoblotting. Thus, it binds to native ZNRD1 protein and should be useful in studies of ZNRD1 protein function and expression. By Western immunoblotting analysis of 16 patients with gastric cancer using the MAb, we found ZNRD1 protein was more overexpressed in incision margin than in carcinoma tissue. The results showed that ZNRD1 might be a novel modifier in gastric carcinogenesis.

Original language	English
Pages (from-to)	65-68
Number of pages	4
Journal	Hybridoma and Hybridomics
Volume	23
Issue number	1
DOIs	https://doi.org/10.1089/153685904322772042
State	Published - Feb 2004
Externally published	Yes

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title = "Preparation and Characterization of a Novel Monoclonal Antibody Specific to Human ZNRD1 Protein",

abstract = "We have efficiently generated the first monoclonal antibody (MAb) against the human ZNRD1 protein, a transcription-associated protein, consisting of two zinc ribbon domains. Hybridomas were screened by indirect enzyme-linked immunosorbent assay (ELISA) using either purified 6 x His-ZNRD1fusion protein or purified 6 x His-OS-9 fusion protein as a control. One MAb named H6 (IgG 1), effective in detecting the recombinant and cellular protein, was characterized by ELISA and Western immunoblotting. Thus, it binds to native ZNRD1 protein and should be useful in studies of ZNRD1 protein function and expression. By Western immunoblotting analysis of 16 patients with gastric cancer using the MAb, we found ZNRD1 protein was more overexpressed in incision margin than in carcinoma tissue. The results showed that ZNRD1 might be a novel modifier in gastric carcinogenesis.",

author = "Liu Hong and Yumei Zhang and Shuang Han and Jin Wang and Yongquan Shi and Yanglin Pan and Na Liu and Xiaoyin Zhang and Daiming Fan",

year = "2004",

month = feb,

doi = "10.1089/153685904322772042",

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T1 - Preparation and Characterization of a Novel Monoclonal Antibody Specific to Human ZNRD1 Protein

AU - Hong, Liu

AU - Zhang, Yumei

AU - Han, Shuang

AU - Wang, Jin

AU - Shi, Yongquan

AU - Pan, Yanglin

AU - Liu, Na

AU - Zhang, Xiaoyin

AU - Fan, Daiming

PY - 2004/2

Y1 - 2004/2

N2 - We have efficiently generated the first monoclonal antibody (MAb) against the human ZNRD1 protein, a transcription-associated protein, consisting of two zinc ribbon domains. Hybridomas were screened by indirect enzyme-linked immunosorbent assay (ELISA) using either purified 6 x His-ZNRD1fusion protein or purified 6 x His-OS-9 fusion protein as a control. One MAb named H6 (IgG 1), effective in detecting the recombinant and cellular protein, was characterized by ELISA and Western immunoblotting. Thus, it binds to native ZNRD1 protein and should be useful in studies of ZNRD1 protein function and expression. By Western immunoblotting analysis of 16 patients with gastric cancer using the MAb, we found ZNRD1 protein was more overexpressed in incision margin than in carcinoma tissue. The results showed that ZNRD1 might be a novel modifier in gastric carcinogenesis.

AB - We have efficiently generated the first monoclonal antibody (MAb) against the human ZNRD1 protein, a transcription-associated protein, consisting of two zinc ribbon domains. Hybridomas were screened by indirect enzyme-linked immunosorbent assay (ELISA) using either purified 6 x His-ZNRD1fusion protein or purified 6 x His-OS-9 fusion protein as a control. One MAb named H6 (IgG 1), effective in detecting the recombinant and cellular protein, was characterized by ELISA and Western immunoblotting. Thus, it binds to native ZNRD1 protein and should be useful in studies of ZNRD1 protein function and expression. By Western immunoblotting analysis of 16 patients with gastric cancer using the MAb, we found ZNRD1 protein was more overexpressed in incision margin than in carcinoma tissue. The results showed that ZNRD1 might be a novel modifier in gastric carcinogenesis.

UR - https://www.scopus.com/pages/publications/1242294514

U2 - 10.1089/153685904322772042

DO - 10.1089/153685904322772042

M3 - 文章

C2 - 15000850

AN - SCOPUS:1242294514

SN - 1536-8599

VL - 23

SP - 65

EP - 68

JO - Hybridoma and Hybridomics

JF - Hybridoma and Hybridomics

IS - 1

ER -

Preparation and Characterization of a Novel Monoclonal Antibody Specific to Human ZNRD1 Protein

Abstract

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