Pharmacokinetics of recombinant human basic fibroblast growth factor in mice using a radioiodination method combined with SDS-PAGE and a sandwich enzyme-linked immunosorbent assay

Q. Zhang; G. J. Wang; J. G. Sun

doi:10.1007/BF03190593

Pharmacokinetics of recombinant human basic fibroblast growth factor in mice using a radioiodination method combined with SDS-PAGE and a sandwich enzyme-linked immunosorbent assay

Q. Zhang
, G. J. Wang
, J. G. Sun

China Pharmaceutical University

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Pharmacokinetics of recombinant human basic fibroblast growth factor (rhbFGF) in mice were studied by using a radioiodination method combined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a sandwich enzyme-Linked immunosorbent assay (ELISA). RhbFGF concentration in serum were dertermined after iv administration of 2.5, 5 and 10 μg/kg of ¹²⁵I-rhbFGF or rhbFGF. The AUCs were linearly correlated to doses. Concentrations in tissues, recoveries of ¹²⁵I-rhbFGF in urine and feces were also analyzed following iv dose of 5 μg/kg. Pharmacokinetic profiles of radiolabelled and unlabelled rhbFGF were consistent in trend but exit certain differences. Mice cleared unlabeled rhbFGF markedly faster than their radiolabeled counterparts.

Original language	English
Pages (from-to)	163-168
Number of pages	6
Journal	European Journal of Drug Metabolism and Pharmacokinetics
Volume	29
Issue number	3
DOIs	https://doi.org/10.1007/BF03190593
State	Published - 2004
Externally published	Yes

Keywords

ELISA
Method difference
Pharmacokinetics
Radioiodination
Recombinant human basic fibroblast growth factor (rhbFGF)

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10.1007/BF03190593

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@article{51cc059f46ab4494a412523c8efa9fa6,

title = "Pharmacokinetics of recombinant human basic fibroblast growth factor in mice using a radioiodination method combined with SDS-PAGE and a sandwich enzyme-linked immunosorbent assay",

abstract = "Pharmacokinetics of recombinant human basic fibroblast growth factor (rhbFGF) in mice were studied by using a radioiodination method combined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a sandwich enzyme-Linked immunosorbent assay (ELISA). RhbFGF concentration in serum were dertermined after iv administration of 2.5, 5 and 10 μg/kg of 125I-rhbFGF or rhbFGF. The AUCs were linearly correlated to doses. Concentrations in tissues, recoveries of 125I-rhbFGF in urine and feces were also analyzed following iv dose of 5 μg/kg. Pharmacokinetic profiles of radiolabelled and unlabelled rhbFGF were consistent in trend but exit certain differences. Mice cleared unlabeled rhbFGF markedly faster than their radiolabeled counterparts.",

keywords = "ELISA, Method difference, Pharmacokinetics, Radioiodination, Recombinant human basic fibroblast growth factor (rhbFGF)",

author = "Q. Zhang and Wang, \{G. J.\} and Sun, \{J. G.\}",

year = "2004",

doi = "10.1007/BF03190593",

language = "英语",

volume = "29",

pages = "163--168",

journal = "European Journal of Drug Metabolism and Pharmacokinetics",

issn = "0378-7966",

publisher = "Adis",

number = "3",

}

Pharmacokinetics of recombinant human basic fibroblast growth factor in mice using a radioiodination method combined with SDS-PAGE and a sandwich enzyme-linked immunosorbent assay. / Zhang, Q.; Wang, G. J.; Sun, J. G.
In: European Journal of Drug Metabolism and Pharmacokinetics, Vol. 29, No. 3, 2004, p. 163-168.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Pharmacokinetics of recombinant human basic fibroblast growth factor in mice using a radioiodination method combined with SDS-PAGE and a sandwich enzyme-linked immunosorbent assay

AU - Zhang, Q.

AU - Wang, G. J.

AU - Sun, J. G.

PY - 2004

Y1 - 2004

N2 - Pharmacokinetics of recombinant human basic fibroblast growth factor (rhbFGF) in mice were studied by using a radioiodination method combined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a sandwich enzyme-Linked immunosorbent assay (ELISA). RhbFGF concentration in serum were dertermined after iv administration of 2.5, 5 and 10 μg/kg of 125I-rhbFGF or rhbFGF. The AUCs were linearly correlated to doses. Concentrations in tissues, recoveries of 125I-rhbFGF in urine and feces were also analyzed following iv dose of 5 μg/kg. Pharmacokinetic profiles of radiolabelled and unlabelled rhbFGF were consistent in trend but exit certain differences. Mice cleared unlabeled rhbFGF markedly faster than their radiolabeled counterparts.

AB - Pharmacokinetics of recombinant human basic fibroblast growth factor (rhbFGF) in mice were studied by using a radioiodination method combined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a sandwich enzyme-Linked immunosorbent assay (ELISA). RhbFGF concentration in serum were dertermined after iv administration of 2.5, 5 and 10 μg/kg of 125I-rhbFGF or rhbFGF. The AUCs were linearly correlated to doses. Concentrations in tissues, recoveries of 125I-rhbFGF in urine and feces were also analyzed following iv dose of 5 μg/kg. Pharmacokinetic profiles of radiolabelled and unlabelled rhbFGF were consistent in trend but exit certain differences. Mice cleared unlabeled rhbFGF markedly faster than their radiolabeled counterparts.

KW - ELISA

KW - Method difference

KW - Pharmacokinetics

KW - Radioiodination

KW - Recombinant human basic fibroblast growth factor (rhbFGF)

UR - https://www.scopus.com/pages/publications/7444240898

U2 - 10.1007/BF03190593

DO - 10.1007/BF03190593

M3 - 文章

C2 - 15543670

AN - SCOPUS:7444240898

SN - 0378-7966

VL - 29

SP - 163

EP - 168

JO - European Journal of Drug Metabolism and Pharmacokinetics

JF - European Journal of Drug Metabolism and Pharmacokinetics

IS - 3

ER -

Pharmacokinetics of recombinant human basic fibroblast growth factor in mice using a radioiodination method combined with SDS-PAGE and a sandwich enzyme-linked immunosorbent assay

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Keywords

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