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Performance analysis of microchip electrophoresis with portable UV absorbance detection system

  • Harbin Institute of Technology

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This paper presents a portable miniaturized integration UV-VIS absorbance detection system for the microchip electrophoresis (MCE). The TOCON_nano high sensitive SiC pre-amplified UV Sensor (Sglux GmbH, Ostendstrasse, Berlin, Germany) is used to measure extremely low UV radiation intensities in the nanowatt scale. This detection system volume is 280 ×200×150 mm3, the weight is less than 2Kg. The PMMA was used as the MCE substrate materials. And the electrophoresis microchannels are fabricated with the CNC milling, have the cross section area of 150μm ×150μm. With this detection system, the detection limit for the Cl- electrophoresis can reach 10-10mol at the indirect UV-VIS detection mode. Two inorganic ions Cl- and SO4 2- could reach the baseline separation in 3 min.

Original languageEnglish
Title of host publicationAdvances in Manufacturing Technology
Pages1883-1886
Number of pages4
DOIs
StatePublished - 2012
Externally publishedYes
Event2nd International Conference on Advanced Design and Manufacturing Engineering, ADME 2012 - Taiyuan, China
Duration: 16 Aug 201218 Aug 2012

Publication series

NameApplied Mechanics and Materials
Volume220-223
ISSN (Print)1660-9336
ISSN (Electronic)1662-7482

Conference

Conference2nd International Conference on Advanced Design and Manufacturing Engineering, ADME 2012
Country/TerritoryChina
CityTaiyuan
Period16/08/1218/08/12

Keywords

  • Baseline separation
  • Microchip electrophoresis(MCE)
  • SiC
  • UV-Vis detection

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