Abstract
Dopamine and cAMP-regulated phosphoprotein (DARPP)-32 is a phosphoprotein that is centrally involved in dopamine signalling pathways. It may play an important role in gastric tumorigenesis. Our aim is to investigate the expression of DARPP-32 protein in colorectal adenocarcinoma. The expression of DARPP-32 was evaluated by immunohistochemical SABC staining and Western blotting. DARPP-32 was specifically localised in colorectal epithelium. The expression of DARPP-32 in colorectal adenocarcinoma tissues 33/42, 78.57% was higher than that in normal colon epithelial tissues (31/60, 51.67%, p < 0.05). There was no significant relationship between the expression of DARPP-32 and the differentiation, metastasis and Dukes' stage of colorectal adenocarcinoma (p > 0.05). Both DARPP-32 and its truncated isoform t-DARPP were overexpressed in colorectal adenocarcinoma (t = 2.306, p = 0.028), while t-DARPP was more frequently detected. These results showed that DARPP-32 may play an important role in the regulation of normal colorectal epithelial biology and carcinogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 58-61 |
| Number of pages | 4 |
| Journal | International Journal of Clinical Practice |
| Volume | 59 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2005 |
| Externally published | Yes |
Keywords
- Carcinogenesis
- Colorectal cancer
- DARPP-32
