Online solid-phase extraction with high-performance liquid chromatography and mass spectrometry for the determination of five tannins in traditional Chinese medicine injections

Meng Sun; Yuanyuan Lin; Jie Zhang; Shaohua Zheng; Sicen Wang

doi:10.1002/jssc.201501037

Online solid-phase extraction with high-performance liquid chromatography and mass spectrometry for the determination of five tannins in traditional Chinese medicine injections

Meng Sun
, Yuanyuan Lin
, Jie Zhang
, Shaohua Zheng
, Sicen Wang

Health Science Center

Xi'an Jiaotong University

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A rapid analytical method based on online solid-phase extraction with high-performance liquid chromatography and mass spectrometry has been established and applied to the determination of tannin compounds that may cause adverse effects in traditional Chinese medicine injections. Different solid-phase extraction sorbents have been compared and the elution buffer was optimized. The performance of the method was verified by evaluation of recovery (≥40%), repeatability (RSD ≤ 6%), linearity (r² ≥ 0.993), and limit of quantification (≤0.35 μg/mL). Five tannin compounds, gallic acid, cianidanol, gallocatechin gallate, ellagic acid, and penta-O-galloylglucose, were identified with concentrations ranging from 3.1-37.4 μg/mL in the analyzed traditional Chinese medicine injections.

Original language	English
Pages (from-to)	889-894
Number of pages	6
Journal	Journal of Separation Science
Volume	39
Issue number	5
DOIs	https://doi.org/10.1002/jssc.201501037
State	Published - 1 Mar 2016

Keywords

High-performance liquid chromatography
Mass spectrometry
Quality control
Solid-phase extraction
Tannins

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10.1002/jssc.201501037

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title = "Online solid-phase extraction with high-performance liquid chromatography and mass spectrometry for the determination of five tannins in traditional Chinese medicine injections",

abstract = "A rapid analytical method based on online solid-phase extraction with high-performance liquid chromatography and mass spectrometry has been established and applied to the determination of tannin compounds that may cause adverse effects in traditional Chinese medicine injections. Different solid-phase extraction sorbents have been compared and the elution buffer was optimized. The performance of the method was verified by evaluation of recovery (≥40\%), repeatability (RSD ≤ 6\%), linearity (r2 ≥ 0.993), and limit of quantification (≤0.35 μg/mL). Five tannin compounds, gallic acid, cianidanol, gallocatechin gallate, ellagic acid, and penta-O-galloylglucose, were identified with concentrations ranging from 3.1-37.4 μg/mL in the analyzed traditional Chinese medicine injections.",

keywords = "High-performance liquid chromatography, Mass spectrometry, Quality control, Solid-phase extraction, Tannins",

author = "Meng Sun and Yuanyuan Lin and Jie Zhang and Shaohua Zheng and Sicen Wang",

note = "Publisher Copyright: {\textcopyright} 2015 Wiley-VCH Verlag GmbH \& Co. KGaA, Weinheim.",

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doi = "10.1002/jssc.201501037",

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TY - JOUR

T1 - Online solid-phase extraction with high-performance liquid chromatography and mass spectrometry for the determination of five tannins in traditional Chinese medicine injections

AU - Sun, Meng

AU - Lin, Yuanyuan

AU - Zhang, Jie

AU - Zheng, Shaohua

AU - Wang, Sicen

PY - 2016/3/1

Y1 - 2016/3/1

N2 - A rapid analytical method based on online solid-phase extraction with high-performance liquid chromatography and mass spectrometry has been established and applied to the determination of tannin compounds that may cause adverse effects in traditional Chinese medicine injections. Different solid-phase extraction sorbents have been compared and the elution buffer was optimized. The performance of the method was verified by evaluation of recovery (≥40%), repeatability (RSD ≤ 6%), linearity (r2 ≥ 0.993), and limit of quantification (≤0.35 μg/mL). Five tannin compounds, gallic acid, cianidanol, gallocatechin gallate, ellagic acid, and penta-O-galloylglucose, were identified with concentrations ranging from 3.1-37.4 μg/mL in the analyzed traditional Chinese medicine injections.

AB - A rapid analytical method based on online solid-phase extraction with high-performance liquid chromatography and mass spectrometry has been established and applied to the determination of tannin compounds that may cause adverse effects in traditional Chinese medicine injections. Different solid-phase extraction sorbents have been compared and the elution buffer was optimized. The performance of the method was verified by evaluation of recovery (≥40%), repeatability (RSD ≤ 6%), linearity (r2 ≥ 0.993), and limit of quantification (≤0.35 μg/mL). Five tannin compounds, gallic acid, cianidanol, gallocatechin gallate, ellagic acid, and penta-O-galloylglucose, were identified with concentrations ranging from 3.1-37.4 μg/mL in the analyzed traditional Chinese medicine injections.

KW - High-performance liquid chromatography

KW - Mass spectrometry

KW - Quality control

KW - Solid-phase extraction

KW - Tannins

UR - https://www.scopus.com/pages/publications/84956867421

U2 - 10.1002/jssc.201501037

DO - 10.1002/jssc.201501037

M3 - 文章

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AN - SCOPUS:84956867421

SN - 1615-9306

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EP - 894

JO - Journal of Separation Science

JF - Journal of Separation Science

IS - 5

ER -

Online solid-phase extraction with high-performance liquid chromatography and mass spectrometry for the determination of five tannins in traditional Chinese medicine injections

Abstract

Keywords

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