TY - JOUR
T1 - Novel hydroxyethyl chitosan/cellulose scaffolds with bubble-like porous structure for bone tissue engineering
AU - Wang, Yaping
AU - Qian, Junmin
AU - Zhao, Na
AU - Liu, Ting
AU - Xu, Weijun
AU - Suo, Aili
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Hydrogels fabricated from natural polysaccharides may serve as ideal scaffolds for tissue engineering because of their similarity to the extracellular matrices. In this study, novel hydrogel scaffolds with bubble-like porous structure were prepared from hydroxyethyl chitosan (HECS) and cellulose (CEL) by a combination of chemical crosslinking, particle-leaching using silicon dioxide particles as porogen and freeze-drying method. The morphology, compression stress-strain curves, wettability, and swelling and rheological behaviors of the HECS/CEL scaffolds were characterized by SEM, mechanical test, contact angle measurement and rheometer. HECS/CEL scaffolds had good comprehensive performances and could reach equilibrium swelling state in water within 20s. The results from in vitro biocompatibility evaluated using SEM, live/dead cell viability and MTT assays demonstrated that the HECS/CEL scaffolds could well support the attachment, spreading and proliferation of osteoblastic MC3T3-E1 cells and showed good biocompatibility. Therefore, the novel HECS/CEL scaffolds may be promising for bone tissue engineering applications.
AB - Hydrogels fabricated from natural polysaccharides may serve as ideal scaffolds for tissue engineering because of their similarity to the extracellular matrices. In this study, novel hydrogel scaffolds with bubble-like porous structure were prepared from hydroxyethyl chitosan (HECS) and cellulose (CEL) by a combination of chemical crosslinking, particle-leaching using silicon dioxide particles as porogen and freeze-drying method. The morphology, compression stress-strain curves, wettability, and swelling and rheological behaviors of the HECS/CEL scaffolds were characterized by SEM, mechanical test, contact angle measurement and rheometer. HECS/CEL scaffolds had good comprehensive performances and could reach equilibrium swelling state in water within 20s. The results from in vitro biocompatibility evaluated using SEM, live/dead cell viability and MTT assays demonstrated that the HECS/CEL scaffolds could well support the attachment, spreading and proliferation of osteoblastic MC3T3-E1 cells and showed good biocompatibility. Therefore, the novel HECS/CEL scaffolds may be promising for bone tissue engineering applications.
KW - Bubble-like porous structure
KW - Cellulose
KW - Hydrogel scaffold
KW - Hydroxyethyl chitosan
KW - In vitro biocompatibility
UR - https://www.scopus.com/pages/publications/85015615838
U2 - 10.1016/j.carbpol.2017.03.030
DO - 10.1016/j.carbpol.2017.03.030
M3 - 文章
C2 - 28433176
AN - SCOPUS:85015615838
SN - 0144-8617
VL - 167
SP - 44
EP - 51
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -