Non-aqueous capillary electrophoresis for separation and simultaneous determination of magnoflorine, taspine, and caulophine in Caulophyllum robustum collected in different seasons

A non-aqueous capillary electrophoresis (NACE) method has been developed for the simultaneous determination of magnoflorine, taspine, and caulophine in Caulophyllum robustum collected in different seasons. The relative standard deviations (RSDs) of the migration times and peak areas of the three components were 0.33 and 3.34% for magnoflorine, 0.45 and 2.67% for taspine, and 0.49 and 3.71% for caulophine, respectively. Detection limits of magnoflorine, taspine, and caulophine were 0.30 μg/mL, 0.08 μg/mL, and 0.10 μg/mL, respectively. This method has been successfully applied to simultaneous determination of the three bioactive components in the different collection seasons.