TY - GEN
T1 - Multifunctional SH-SY5Y-based biomimetic sensor for integrated detection of olfaction, gustation and toxicity
AU - Gao, Keqiang
AU - Gao, Fan
AU - Du, Liping
AU - He, Chuanjiang
AU - Wan, Hao
AU - Wang, Ping
N1 - Publisher Copyright:
© 2019 IEEE.
PY - 2019/5
Y1 - 2019/5
N2 - The detection of olfaction, gustation and toxicity has played an important role in industries and research. The key factor to realize this function is to detect different substances with the same sensitive element. In this paper, the human neuroblastoma SH-SY5Y cells endogenously expressed the human bitter receptor, T2R16. Meanwhile, an olfactory receptor, ODR-10, was transfected on the plasma membrane of the cells at the same time. T2R16 could specifically respond to bitter compounds, which can be monitored by cell-impedance sensor. The ODR-10 receptor can also specifically respond to diacetyl, which was cultured on the microelectrode arrays to establish a novel biomimetic sensor for odor detection. The results represent a dose-dependent responses in a certain concentration range. Moreover, the cell-impedance biosensor enabled a quick toxicity detection of salicin. In conclusion, the biomimetic sensors based on extracellular recording in vitro show great potential for use in both basic research and practical applications.
AB - The detection of olfaction, gustation and toxicity has played an important role in industries and research. The key factor to realize this function is to detect different substances with the same sensitive element. In this paper, the human neuroblastoma SH-SY5Y cells endogenously expressed the human bitter receptor, T2R16. Meanwhile, an olfactory receptor, ODR-10, was transfected on the plasma membrane of the cells at the same time. T2R16 could specifically respond to bitter compounds, which can be monitored by cell-impedance sensor. The ODR-10 receptor can also specifically respond to diacetyl, which was cultured on the microelectrode arrays to establish a novel biomimetic sensor for odor detection. The results represent a dose-dependent responses in a certain concentration range. Moreover, the cell-impedance biosensor enabled a quick toxicity detection of salicin. In conclusion, the biomimetic sensors based on extracellular recording in vitro show great potential for use in both basic research and practical applications.
KW - Biomimetic sensors
KW - MEAs
KW - SH-SY5Y
KW - bitter and toxic compounds detection
KW - cell-impedance
KW - odor
UR - https://www.scopus.com/pages/publications/85072956153
U2 - 10.1109/ISOEN.2019.8823301
DO - 10.1109/ISOEN.2019.8823301
M3 - 会议稿件
AN - SCOPUS:85072956153
T3 - ISOEN 2019 - 18th International Symposium on Olfaction and Electronic Nose, Proceedings
BT - ISOEN 2019 - 18th International Symposium on Olfaction and Electronic Nose, Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 18th International Symposium on Olfaction and Electronic Nose, ISOEN 2019
Y2 - 26 May 2019 through 29 May 2019
ER -