TY - JOUR
T1 - Mitochondrial decay in the brains of old rats
T2 - Ameliorating effect of alpha-lipoic acid and acetyl-L-carnitine
AU - Long, Jiangang
AU - Gao, Feng
AU - Tong, Liqi
AU - Cotman, Carl W.
AU - Ames, Bruce N.
AU - Liu, Jiankang
PY - 2009/4
Y1 - 2009/4
N2 - To investigate the mitochondrial decay and oxidative damage resulting from aging, the activities/kinetics of the mitochondrial complexes were examined in the brains of young and old rats as well as in old rats fed R-α-lipoic acid plus acetyl-l-carnitine (LA/ALC). The brain mitochondria of old rats, compared with young rats, had significantly decreased endogenous antioxidants and superoxide dismutase activity; more oxidative damage to lipids and proteins; and decreased activities of complex I, IV and V. Complex I showed a decrease in binding affinity (increase in Km) for substrates. Feeding LA/ALC to old rats partially restored age-associated mitochondrial dysfunction to the levels of the young rats. These results indicate that oxidative mitochondrial decay plays an important role in brain aging and that a combination of nutrients targeting mitochondria, such as LA/ALC, could ameliorate mitochondrial decay through preventing mitochondrial oxidative damage.
AB - To investigate the mitochondrial decay and oxidative damage resulting from aging, the activities/kinetics of the mitochondrial complexes were examined in the brains of young and old rats as well as in old rats fed R-α-lipoic acid plus acetyl-l-carnitine (LA/ALC). The brain mitochondria of old rats, compared with young rats, had significantly decreased endogenous antioxidants and superoxide dismutase activity; more oxidative damage to lipids and proteins; and decreased activities of complex I, IV and V. Complex I showed a decrease in binding affinity (increase in Km) for substrates. Feeding LA/ALC to old rats partially restored age-associated mitochondrial dysfunction to the levels of the young rats. These results indicate that oxidative mitochondrial decay plays an important role in brain aging and that a combination of nutrients targeting mitochondria, such as LA/ALC, could ameliorate mitochondrial decay through preventing mitochondrial oxidative damage.
KW - Binding affinity (K)
KW - Brain mitochondria
KW - Enzyme kinetics
KW - Mitochondrial complex activity
KW - Oxidative damage
UR - https://www.scopus.com/pages/publications/62349088898
U2 - 10.1007/s11064-008-9850-2
DO - 10.1007/s11064-008-9850-2
M3 - 文章
C2 - 18846423
AN - SCOPUS:62349088898
SN - 0364-3190
VL - 34
SP - 755
EP - 763
JO - Neurochemical Research
JF - Neurochemical Research
IS - 4
ER -