miR-200b对三阴性乳腺癌细胞MDA-MB-231的负性调控作用

Objective: To identify the regulation of miRNA-200b on proliferation of triple negative breast cancer cells. Methods: The expressions of miRNA-200a/b/c in breast cancer cell lines MCF-7, BT-474, MDA-MB-453, SKBR3, MDA-MB-468, and MDA-MB-231 were detected by real-time PCR. MTT was used to detect MDA-MB-231 cell proliferation after miR-200b was downregulated and upregulated. The colony-forming efficiency of miR-200b was detected by colony forming assay. The transwell chamber was used to investigate the influence of miR-200b on invasion of MDA-MB-231 cells. Results: miRNA-200a/b/c had relatively low expressions in triple negative breast cancer cell lines MDA-MB-468 and MDA-MB-231, but high expressions in luminal cells MCF-7, BT-474, and Her-2 positive cells MDA-MB-453, SKBR3. The upregulated miR-200b could suppress the proliferation, colony formation and cell invasion of MDA-MB-231 cells (P<0.05). Also the downregulated miR-200b promoted cell proliferation, colony forming and cell invasion (P<0.05). Conclusion: miRNA-200b suppresses triple negative breast cancer cell MDA-MB-231, making it a potential biomarker and target for early diagnosis and therapy.